Project description:The Madin-Darby bovine kidney epithelial cells, when at approximately 50% confluence (during the exponential phase), were treated with 10mM sodium butyrate for 24h. Butyrate induced profound changes in gene expression related with multiple signal transduction pathways such as cell cycle control, apoptosis and extracellular matrix remodeling in this cell line Keywords: stress response, two class unpaired design
Project description:The expression of TGFbeta pathway genes were downregulated in HIF1A KD Madin-Darby canine kidney epithelial cells (MDCKII) in hypoxic condition. We used microarray to study the differential expression of genes in HIF1A knockdown versus WT Madin-Darby canine kidney epithelial cells (MDCKII)
Project description:No one pathway was identified in up- and downregulated genes profile in HIF2A KD Madin-Darby canine kidney epithelial cells (MDCKII) in normoxic condition. We used microarray to study the differential expression of genes in HIF2A knockdown versus WT Madin-Darby canine kidney epithelial cells (MDCKII)
Project description:In order to further understanding the replication and pathogenesis of Caprine herpesvirus 1 (CpHV-1) and virus-host interactions. In this study, the proteomics of CpHV-1 infected Madin Darby bovine kidney (MDBK) cells was explored by using iTRAQ-LC-MS/MS technology.
Project description:Cultured Madin-Darby canine kidney (MDCK) cells treated with hepatocyte growth factor (HGF) for 0, 3 and 24 hours. Each time point in triplicate. Keywords: time-course
Project description:In this work, changes in the proteome level during a step-wise adaptation of an adherent Madin Darby canine kidney (MDCK) cell line to suspension growth and chemically defined medium were analyzed.
Project description:To investigate whether and how the presence of RasV12-transformed cells influences the gene expression profile of the neighbouring normal epithelial cells, we performed microarray analysis. Normal Madin-Darby canine kidney (MDCK) cells were co-cultured with MDCK cells expressing GFP or GFP-RasV12. GFP-negative normal MDCK cells were then collected by FACS, and expression of various genes was compared between the two conditions.
