Proteomics of mouse liver microsomes: performance of different protein separation workflows for LC-MS/MS
Ontology highlight
ABSTRACT:
INSTRUMENT(S): instrument model, Agilent instrument model
ORGANISM(S): Mus Musculus (mouse)
TISSUE(S): Liver
SUBMITTER: 
Victor Zgoda
PROVIDER: PRD000089 | Pride | 2009-07-28
REPOSITORIES: Pride
Dataset's files
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Publications
Proteomics of mouse liver microsomes: performance of different protein separation workflows for LC-MS/MS.
Zgoda Victor G VG Moshkovskii Sergei A SA Ponomarenko Elena A EA Andreewski Timofey V TV Kopylov Arthur T AT Tikhonova Olga V OV Melnik Stanislav A SA Lisitsa Andrei V AV Archakov Alexander I AI
Proteomics 20090801 16
The mouse liver microsome proteome was investigated using ion trap MS combined with three separation workflows including SDS-PAGE followed by reverse-phase LC of in-gel protein digestions (519 proteins identified); 2-D LC of protein digestion (1410 proteins); whole protein separation on mRP heat-stable column followed by 2-D LC of protein digestions from each fraction (3-D LC; 3703 proteins). The higher number of proteins identified in the workflow corresponded to the lesser percentage of run-to ...[more]
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