Dataset Information
Quantification of tyrosine phosphorylation after EGF stimulation
Ontology highlight
ABSTRACT:
INSTRUMENT(S): LTQ Orbitrap, instrument model
ORGANISM(S): Homo Sapiens (human)
TISSUE(S): Hela Cell
SUBMITTER: 
Paul Boersema
PROVIDER: PRD000124 | Pride | 2010-07-09
REPOSITORIES: Pride
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| PRIDE_Exp_Complete_Ac_9778.pride.mgf.gz | Mgf | |||
| PRIDE_Exp_Complete_Ac_9778.pride.mztab.gz | Mztab | |||
| PRIDE_Exp_Complete_Ac_9778.xml.gz | Xml |
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Publications
In-depth qualitative and quantitative profiling of tyrosine phosphorylation using a combination of phosphopeptide immunoaffinity purification and stable isotope dimethyl labeling.
Molecular & cellular proteomics : MCP 20090921 1
Several mass spectrometry-based assays have emerged for the quantitative profiling of cellular tyrosine phosphorylation. Ideally, these methods should reveal the exact sites of tyrosine phosphorylation, be quantitative, and not be cost-prohibitive. The latter is often an issue as typically several milligrams of (stable isotope-labeled) starting protein material are required to enable the detection of low abundance phosphotyrosine peptides. Here, we adopted and refined a peptidecentric immunoaffi ...[more]
