Proteomics

Dataset Information

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Comparison of the Membrane Proteomics Initiative Standard (MPIS) with the Precursor Microsomes

ABSTRACT: Precursor microsomes (MPIS). In-solution tryptic digest with 6 replicates (2 different concentrations and top5 versus top10)

INSTRUMENT(S): instrument model, LTQ

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Liver

SUBMITTER: Eugene Kapp  

PROVIDER: PRD000176 | Pride | 2010-06-08

REPOSITORIES: Pride

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Dataset's files

Source:
Action DRS
PRIDE_Exp_Complete_Ac_10632.pride.mgf.gz Mgf
PRIDE_Exp_Complete_Ac_10632.pride.mztab.gz Mztab
PRIDE_Exp_Complete_Ac_10632.xml.gz Xml
PRIDE_Exp_Complete_Ac_10633.pride.mgf.gz Mgf
PRIDE_Exp_Complete_Ac_10633.pride.mztab.gz Mztab
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Publications

The Asia Oceania Human Proteome Organisation Membrane Proteomics Initiative. Preparation and characterisation of the carbonate-washed membrane standard.

Peng Lifeng L   Kapp Eugene A EA   Fenyö David D   Kwon Min-Seok MS   Jiang Pu P   Wu Songfeng S   Jiang Ying Y   Aguilar Marie-Isabel MI   Ahmed Nikhat N   Baker Mark S MS   Cai Zongwei Z   Chen Yu-Ju YJ   Van Chi Phan P   Chung Maxey C M MC   He Fuchu F   Len Alice C L AC   Liao Pao-Chi PC   Nakamura Kazuyuki K   Ngai Sai Ming SM   Paik Young-Ki YK   Pan Tai-Long TL   Poon Terence C W TC   Salekdeh Ghasem Hosseini GH   Simpson Richard J RJ   Sirdeshmukh Ravi R   Srisomsap Chantragan C   Svasti Jisnuson J   Tyan Yu-Chang YC   Dreyer Florian S FS   McLauchlan Danyl D   Rawson Pisana P   Jordan T William TW  

Proteomics 20101101 22

The Asia Oceania Human Proteome Organisation (AOHUPO) has embarked on a Membrane Proteomics Initiative with goals of systematic comparison of strategies for analysis of membrane proteomes and discovery of membrane proteins. This multilaboratory project is based on the analysis of a subcellular fraction from mouse liver that contains endoplasmic reticulum and other organelles. In this study, we present the strategy used for the preparation and initial characterization of the membrane sample, incl  ...[more]

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