Dataset Information

Identification of temperature stress response proteins in suspension cultured cells of Oryza sativa (cultivar Doongara)

ABSTRACT: Not available

INSTRUMENT(S): instrument model, LTQ XL

ORGANISM(S): O. Sativa Indica Group

SUBMITTER: C. Gayani Gammulla

PROVIDER: PRD000183 | Pride | 2010-09-14

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	PRIDE_Exp_Complete_Ac_10726.pride.mztab.gz	Mztab
	PRIDE_Exp_Complete_Ac_10726.xml.gz	Xml
	PRIDE_Exp_Complete_Ac_10727.pride.mztab.gz	Mztab
	PRIDE_Exp_Complete_Ac_10727.xml.gz	Xml
	PRIDE_Exp_Complete_Ac_10728.pride.mztab.gz	Mztab

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Publications

Differential metabolic response of cultured rice (Oryza sativa) cells exposed to high- and low-temperature stress.

Gammulla Chumithri Gayani CG Pascovici Dana D Atwell Brian J BJ Haynes Paul A PA

Proteomics 20100801 16

Global mean temperatures are expected to rise by 2-4.5 degrees C by 2100, accompanied by an increase in frequency and amplitude of extreme temperature events. Greater climatic extremes and an expanded range of cultivation will expose rice to increasing stress in the future. Understanding gene expression in disparate thermal regimes is important for the engineering of cultivars with tolerance to nonoptimal temperatures. Our study investigated the proteomic responses of rice cell suspension cultur ...[more]

PMID: 20645384

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Project description:BackgroundRice yield and quality are adversely affected by high temperatures, especially at night; high nighttime temperatures are more harmful to grain weight than high daytime temperatures. Unfortunately, global temperatures are consistently increasing at an alarming rate and the minimum nighttime temperature has increased three times as much as the corresponding maximum daytime temperature over the past few decades.ResultsWe analyzed the transcriptome profiles for rice grain from heat-tolerant and -sensitive lines in response to high night temperatures at the early milky stage using the Illumina Sequencing method. The analysis results for the sequencing data indicated that 35 transcripts showed different expressions between heat-tolerant and -sensitive rice, and RT-qPCR analyses confirmed the expression patterns of selected transcripts. Functional analysis of the differentially expressed transcripts indicated that 21 genes have functional annotation and their functions are mainly involved in oxidation-reduction (6 genes), metabolic (7 genes), transport (4 genes), transcript regulation (2 genes), defense response (1 gene) and photosynthetic (1 gene) processes. Based on the functional annotation of the differentially expressed genes, the possible process that regulates these differentially expressed transcripts in rice grain responding to high night temperature stress at the early milky stage was further analyzed. This analysis indicated that high night temperature stress disrupts electron transport in the mitochondria, which leads to changes in the concentration of hydrogen ions in the mitochondrial and cellular matrix and influences the activity of enzymes involved in TCA and its secondary metabolism in plant cells.ConclusionsUsing Illumina sequencing technology, the differences between the transcriptomes of heat-tolerant and -sensitive rice lines in response to high night temperature stress at the early milky stage was described here for the first time. The candidate transcripts may provide genetic resources that may be useful in the improvement of heat-tolerant characters of rice. The model proposed here is based on differences in expression and transcription between two rice lines. In addition, the model may support future studies on the molecular mechanisms underlying plant responses to high night temperatures.