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Low ORP anaerobiosis - Expanding the known repertoire of virulence factors produced by Bacillus cereus through early secretome profiling in three redox conditions

ABSTRACT: shotgun comparison of aerobiosis and high and low ORP anaerobiosis

INSTRUMENT(S): LTQ Orbitrap, instrument model

ORGANISM(S): Bacillus Cereus (strain Atcc 14579 / Dsm 31)

SUBMITTER: Jean ARMENGAUD

PROVIDER: PRD000192 | Pride | 2011-05-05

REPOSITORIES: Pride

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			Action	DRS
	PRIDE_Exp_Complete_Ac_11343.pride.mgf.gz	Mgf
	PRIDE_Exp_Complete_Ac_11343.pride.mztab.gz	Mztab
	PRIDE_Exp_Complete_Ac_11343.xml.gz	Xml
	PRIDE_Exp_Complete_Ac_11344.pride.mgf.gz	Mgf
	PRIDE_Exp_Complete_Ac_11344.pride.mztab.gz	Mztab

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Publications

Expanding the known repertoire of virulence factors produced by Bacillus cereus through early secretome profiling in three redox conditions.

Clair Gérémy G Roussi Stamatiki S Armengaud Jean J Duport Catherine C

Molecular & cellular proteomics : MCP 20100405 7

The pathogen Bacillus cereus causes diarrheal disease in humans. In the small intestine, B. cereus has to deal with anaerobiosis, low oxidoreduction potential, and carbohydrate limitation conditions. To gain insight into the virulence potential of low density B. cereus cells in such an environment, we cultured bacteria in low and high oxidoreduction potential anoxic conditions and in fully oxic conditions and compared their full secretomes. A unique pattern of proteins assigned to virulence fact ...[more]

PMID: 20368289

Similar Datasets

high ORP anaerobiosis - Expanding the known repertoire of virulence factors produced by Bacillus cereus through early secretome profiling in three redox conditions

Project description:shotgun comparison of aerobiosis to low and high ORP anaerobiosis

2011-05-05 | PRD000191 | Pride

full aerobiosis - Expanding the known repertoire of virulence factors produced by Bacillus cereus through early secretome profiling in three redox conditions

Project description:shotgun comparison of aerobiosis to low and high ORP anaerobiosis

2011-05-05 | PRD000193 | Pride

Expanding the known repertoire of virulence factors produced by Bacillus cereus through early secretome profiling in three redox conditions.

Project description:The pathogen Bacillus cereus causes diarrheal disease in humans. In the small intestine, B. cereus has to deal with anaerobiosis, low oxidoreduction potential, and carbohydrate limitation conditions. To gain insight into the virulence potential of low density B. cereus cells in such an environment, we cultured bacteria in low and high oxidoreduction potential anoxic conditions and in fully oxic conditions and compared their full secretomes. A unique pattern of proteins assigned to virulence factors was revealed. Among the 57 virulence-related factors, 31 were found for the first time in the B. cereus secretome. The putative fourth component of hemolysin BL (HblB'), enterotoxin FM, hemolysin II, and three new putative conserved enterotoxins were uncovered. Cross-comparison of the relative abundance of secreted proteins reveals that a restricted set comprising 19 proteins showed significant changes in response to redox condition changes. We complemented these results with transcriptomics data and confirmed the cytotoxicity of the B. cereus secretome toward Caco-2 human epithelial cells. Our data suggest that (i) the redox-dependent regulatory pathway may modulate the expression of a subset of virulence factors to ensure an appropriate response in a specific redox environment, and (ii) an early growth phase-dependent pathway could regulate the expression of several virulence factors, allowing B. cereus to infect a host whatever the redox conditions. This early growth phase-dependent pathway may function, at least partially, independently of the pleiotropic virulence gene regulator PlcR and may therefore be more specific to the B. cereus group.

| S-EPMC2938089 | biostudies-literature

Bacillus cereus proteomics - Proteomic evidences for rex regulation of metabolism in toxin-producing Bacillus cereus ATCC 14579

Project description:This project contributes to the proteomic comparison of Bacillus cereus wild-type and Bacillus cereus mutant devoided of the Rex regulator. Aerobiosis and anaerobiosis conditions were assayed.

2016-07-09 | PXD000856 | Pride

Bacillus cereus proteomics - Proteomic Evidences for Rex Regulation of Metabolism in Toxin-Producing Bacillus cereus ATCC 14579

2014-09-25 | PXD000886 | Pride

Adaptation of Listeria weihenstephanensis to anaerobiosis

Project description:Adaptation of Listeria weihenstephanensis to anaerobiosis. Trascriptional profiling at two different temperatures of L. weihenstephanensis

2017-08-23 | GSE94124 | GEO

Deciphering the transcriptional adaptation of Corynebacterium glutamicum in transition from aerobiosis via microaerobiosis to anaerobiosis via RNA-seq

Project description:To investigate the adaptation of Corynebacterium glutamicum to altering oxygen availabilities, we conceived a triple-phase fermentation process that describes a gradual reduction of dissolved oxygen depicting a shift from aerobiosis via microaerobiosis to anaerobiosis. The distinct process phases were clearly bordered by the bacteria’s physiologic response such as reduced growth rate, biomass substrate yield and altered yield of fermentation products. During the process, sequential samples were drawn at six points and analyzed via RNA-sequencing of Illumina TruSeq Stranded mRNA libaries sequenced paired end on an Illumina MiSeq system using 75 nt read length. We found transcriptional alterations of almost 50 % (1421 genes) of the entire protein coding genes and observed an upregulation of fermentative pathways, a rearrangement of respiration and mitigation of the basic cellular mechanisms such as transcription, translation and replication as a transient response related to the installed oxygen dependent process phases.

2018-03-31 | E-MTAB-6602 | biostudies-arrayexpress

JM43_Galactose_N2_AntimycinA

Project description:In previous temporal studies, we found the anaerobic response was biphasic when cells growing in galactose medium were shifted from aerobiosis to anaerobiosis, consisting of an acute, transitory phase (<60 min) followed by a more chronic but delayed phase (> 1 generation), but largely monophasic (delayed, chronic phase only) when cells were shifted in glucose medium. Gene network and functional analyses revealed the acute phase was comprised of genes associated with the retooling of metabolism (respiro-fermentative to strictly fermentative) and balancing energy supply and demand. A similar pattern of gene expression is seen when cells encounter other “environmentally stressful” conditions. However, cells shifted to anaerobiosis on glucose, in which no catabolic rearrangement is required nor change in growth rate observed, do not exhibit this pattern, suggesting the “stress” encountered is one associated with the abrupt cessation of galactose-dependent respiration and slowing of growth, not oxygen deprivation per se. In order to test this hypothesis, we added the respiratory inhibitor, antimycin A, under aerobiosis for three generations then shifted to anaerobiosis, which no catabolic rearrangement is required. Keywords: time course

2008-12-31 | GSE3706 | GEO

Tandem mass spectrometry analysis of the thiol proteome from Bacillus cereus grown in anaerobiosisTandem mass spectrometry analysis of the thiol proteome from Bacillus cereus grown in anaerobiosis

Project description:Tandem mass spectrometry analysis of Bacillus cereus samples grown in anaerobiosis for identifying the redox state of the thiols.

2022-02-16 | PXD022049 | Pride

Deciphering the transcriptional adaptation of Corynebacterium glutamicum in transition from aerobiosis via microaerobiosis to anaerobiosis via RNA-seq

Project description:Deciphering the transcriptional adaptation of Corynebacterium glutamicum in transition from aerobiosis via microaerobiosis to anaerobiosis via RNA-seq

| PRJEB25572 | ENA

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