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Full aerobiosis - Expanding the known repertoire of virulence factors produced by Bacillus cereus through early secretome profiling in three redox conditions

ABSTRACT: shotgun comparison of aerobiosis to low and high ORP anaerobiosis

INSTRUMENT(S): LTQ Orbitrap, instrument model

ORGANISM(S): Bacillus Cereus (strain Atcc 14579 / Dsm 31)

SUBMITTER: Jean ARMENGAUD

PROVIDER: PRD000193 | Pride | 2011-05-05

REPOSITORIES: Pride

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			Action	DRS
	PRIDE_Exp_Complete_Ac_11347.pride.mgf.gz	Mgf
	PRIDE_Exp_Complete_Ac_11347.pride.mztab.gz	Mztab
	PRIDE_Exp_Complete_Ac_11347.xml.gz	Xml
	PRIDE_Exp_Complete_Ac_11348.pride.mgf.gz	Mgf
	PRIDE_Exp_Complete_Ac_11348.pride.mztab.gz	Mztab

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Publications

Expanding the known repertoire of virulence factors produced by Bacillus cereus through early secretome profiling in three redox conditions.

Clair Gérémy G Roussi Stamatiki S Armengaud Jean J Duport Catherine C

Molecular & cellular proteomics : MCP 20100405 7

The pathogen Bacillus cereus causes diarrheal disease in humans. In the small intestine, B. cereus has to deal with anaerobiosis, low oxidoreduction potential, and carbohydrate limitation conditions. To gain insight into the virulence potential of low density B. cereus cells in such an environment, we cultured bacteria in low and high oxidoreduction potential anoxic conditions and in fully oxic conditions and compared their full secretomes. A unique pattern of proteins assigned to virulence fact ...[more]

PMID: 20368289

Similar Datasets

low ORP anaerobiosis - Expanding the known repertoire of virulence factors produced by Bacillus cereus through early secretome profiling in three redox conditions

Project description:shotgun comparison of aerobiosis and high and low ORP anaerobiosis

2011-05-05 | PRD000192 | Pride

high ORP anaerobiosis - Expanding the known repertoire of virulence factors produced by Bacillus cereus through early secretome profiling in three redox conditions

Project description:shotgun comparison of aerobiosis to low and high ORP anaerobiosis

2011-05-05 | PRD000191 | Pride

Expanding the known repertoire of virulence factors produced by Bacillus cereus through early secretome profiling in three redox conditions.

Project description:The pathogen Bacillus cereus causes diarrheal disease in humans. In the small intestine, B. cereus has to deal with anaerobiosis, low oxidoreduction potential, and carbohydrate limitation conditions. To gain insight into the virulence potential of low density B. cereus cells in such an environment, we cultured bacteria in low and high oxidoreduction potential anoxic conditions and in fully oxic conditions and compared their full secretomes. A unique pattern of proteins assigned to virulence factors was revealed. Among the 57 virulence-related factors, 31 were found for the first time in the B. cereus secretome. The putative fourth component of hemolysin BL (HblB'), enterotoxin FM, hemolysin II, and three new putative conserved enterotoxins were uncovered. Cross-comparison of the relative abundance of secreted proteins reveals that a restricted set comprising 19 proteins showed significant changes in response to redox condition changes. We complemented these results with transcriptomics data and confirmed the cytotoxicity of the B. cereus secretome toward Caco-2 human epithelial cells. Our data suggest that (i) the redox-dependent regulatory pathway may modulate the expression of a subset of virulence factors to ensure an appropriate response in a specific redox environment, and (ii) an early growth phase-dependent pathway could regulate the expression of several virulence factors, allowing B. cereus to infect a host whatever the redox conditions. This early growth phase-dependent pathway may function, at least partially, independently of the pleiotropic virulence gene regulator PlcR and may therefore be more specific to the B. cereus group.

| S-EPMC2938089 | biostudies-literature

Bacillus cereus proteomics - Proteomic evidences for rex regulation of metabolism in toxin-producing Bacillus cereus ATCC 14579

Project description:This project contributes to the proteomic comparison of Bacillus cereus wild-type and Bacillus cereus mutant devoided of the Rex regulator. Aerobiosis and anaerobiosis conditions were assayed.

2016-07-09 | PXD000856 | Pride

Bacillus cereus proteomics - Proteomic Evidences for Rex Regulation of Metabolism in Toxin-Producing Bacillus cereus ATCC 14579

2014-09-25 | PXD000886 | Pride

JM43_Galactose_N2_AntimycinA

Project description:In previous temporal studies, we found the anaerobic response was biphasic when cells growing in galactose medium were shifted from aerobiosis to anaerobiosis, consisting of an acute, transitory phase (<60 min) followed by a more chronic but delayed phase (> 1 generation), but largely monophasic (delayed, chronic phase only) when cells were shifted in glucose medium. Gene network and functional analyses revealed the acute phase was comprised of genes associated with the retooling of metabolism (respiro-fermentative to strictly fermentative) and balancing energy supply and demand. A similar pattern of gene expression is seen when cells encounter other “environmentally stressful” conditions. However, cells shifted to anaerobiosis on glucose, in which no catabolic rearrangement is required nor change in growth rate observed, do not exhibit this pattern, suggesting the “stress” encountered is one associated with the abrupt cessation of galactose-dependent respiration and slowing of growth, not oxygen deprivation per se. In order to test this hypothesis, we added the respiratory inhibitor, antimycin A, under aerobiosis for three generations then shifted to anaerobiosis, which no catabolic rearrangement is required. Keywords: time course

2008-12-31 | GSE3706 | GEO

The soluble guanylate cyclase CYG12 is required for the acclimation to hypoxia and trophic regimes in Chlamydomonas reinhardtii

Project description:We studied the role of the soluble guanylate cyclase CYG12 in the response of Chlamydomonas reinhardtii to anoxia and darkness. To gain insights into potentially disturbed signaling pathways resulting in different responses of the transcriptome, we performed an RNA-sequencing study to obtain transcriptome profiles of the C. reinhardtii wild-type and a CYG12 knockdown strain in aerobiosis and anaerobiosis in darkness compared to normoxia in the light

2017-11-22 | GSE98442 | GEO

JM43 cells grown aerobically in galactose and treated with Antimycin A

Project description:In previous temporal studies, we found the anaerobic response was biphasic when cells growing in galactose medium were shifted from aerobiosis to anaerobiosis, consisting of an acute, transitory phase (<60 min) followed by a more chronic but delayed phase (> 1 generation), but largely monophasic (delayed, chronic phase only) when cells were shifted in glucose medium. Gene network and functional analyses revealed the acute phase was comprised of genes associated with the retooling of metabolism (respiro-fermentative to strictly fermentative) and balancing energy supply and demand. A similar pattern of gene expression is seen when cells encounter other “environmentally stressful” conditions. However, cells shifted to anaerobiosis on glucose, in which no catabolic rearrangement is required nor change in growth rate observed, do not exhibit this pattern, suggesting the “stress” encountered is one associated with the abrupt cessation of galactose-dependent respiration and slowing of growth, not oxygen deprivation per se. In order to test this hypothesis, we conducted this genomic study using the respiratory inhibitor, antimycin A, under aerobiosis, which mimicked the retooling of metabolism from respiro-fermentative to strictly fermentative growth. Keywords: time course

2008-12-31 | GSE3705 | GEO

JM43_Galactose_N2_AntimycinA

2008-12-31 | E-GEOD-3706 | biostudies-arrayexpress

Bacillus cereus proteomics (Exoproteome)

Project description:This project contributes to the proteomic comparison of Bacillus cereus wild-type and Bacillus cereus mutant devoided of the EntD protein grown in aerobiosis condition and harvested at three growth stages.

2016-06-29 | PXD001482 | Pride

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