Dataset Information

Proteins of Pseudomonas syringae Lz4W

ABSTRACT: Proteins separated on 12%SDS gel, digested with trypsin after cutting the bands, the extracted Peptides were subjected to LC MS/MS on an Ion trap mass spectrometer (Finnigan LTQ from Thermo Electron Corporation).

INSTRUMENT(S): instrument model, LTQ

ORGANISM(S): Pseudomonas Sp.

SUBMITTER: Medicharla V. Jagannadham

PROVIDER: PRD000239 | Pride | 2011-03-29

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	PRIDE_Exp_Complete_Ac_12290.pride.mgf.gz	Mgf
	PRIDE_Exp_Complete_Ac_12290.xml.gz	Xml

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Publications

Identification of outer membrane proteins from an Antarctic bacterium Pseudomonas syringae Lz4W.

Jagannadham M V MV Abou-Eladab Ehab F EF Kulkarni Heramb M HM

Molecular & cellular proteomics : MCP 20110329 6

Subcellular fractionation of proteins is a preferred method of choice for detection and identification of proteins from complex mixtures such as bacterial cells. To characterize the membrane proteins of the Antarctic bacterium Pseudomonas syringae Lz4W, the membrane fractions were prepared using three different methods, namely Triton X-100 solubilization, sucrose density gradient, and carbonate extraction methods. The proteins were separated on one-dimensional polyacrylamide gels and analyzed us ...[more]

PMID: 21447709

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