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P. aeruginosa OMV proteome - Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa

ABSTRACT: Pseudomonas aeruginosa, an opportunistic human bacterial pat hogen, constitutively secretes outer membrane vesicles (OMVs ) into the extracellular milieu. In this study, we identifi ed vesicular proteins with high confidence by five separate LC-MS/MS analyses.

INSTRUMENT(S): instrument model, LTQ XL

ORGANISM(S): Pseudomonas Aeruginosa

SUBMITTER: Yong Song Gho

PROVIDER: PRD000293 | Pride | 2011-08-11

REPOSITORIES: Pride

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			Action	DRS
	PRIDE_Exp_Complete_Ac_13638.pride.mgf.gz	Mgf
	PRIDE_Exp_Complete_Ac_13638.pride.mztab.gz	Mztab
	PRIDE_Exp_Complete_Ac_13638.xml.gz	Xml
	PRIDE_Exp_Complete_Ac_16657.pride.mgf.gz	Mgf
	PRIDE_Exp_Complete_Ac_16657.pride.mztab.gz	Mztab

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Publications

Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.

Choi Dong-Sic DS Kim Dae-Kyum DK Choi Seng Jin SJ Lee Jaewook J Choi Jun-Pyo JP Rho Sangchul S Park Sang-Hyun SH Kim Yoon-Keun YK Hwang Daehee D Gho Yong Song YS

Proteomics 20110801 16

Pseudomonas aeruginosa, an opportunistic human bacterial pathogen, constitutively secretes outer membrane vesicles (OMVs) into the extracellular milieu. Although recent progress has revealed that OMVs are essential for pathogenesis of P. aeruginosa, their proteins have not been comprehensively analyzed so far. In this study, we identified 338 vesicular proteins with high confidence by five separate LC-MS/MS analyses. This global proteome profile provides a basis for future studies to elucidate t ...[more]

PMID: 21751344

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short RNA-Seq of Pseudomonas aeruginosa outer membrane vesicles (OMVs) and whole cells

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small RNA-Seq showing transfer of bacterial outer membrane vesicle (OMV) sRNA to host airway epithelial cells

Project description:This study is the first to show transfer of regulatory bacterial sRNAs from bacterial OMVs to host cells. Demonstration of transfer of bacterial sRNA from Pseudomonas aeruginosa OMVs to host cells in two cell types. RNA isolated from PA14 OMV exposed primary human bronchial epithelial cells or CFBE41o- bronchial epithelial cells as well as unexposed control cells was analyzed by small RNA-Seq. Primary cell exposures were performed on two donors and exposures of CFBE41o- cells were done in triplicate.

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short RNA-Seq of Pseudomonas aeruginosa outer membrane vesicles (OMVs) and whole cells

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Proteomic analysis of Pseudomonas aeruginosa purified outer membrane vesicle (OMV) extracts exposed to sex steroids

Project description:The project aims to elucidate mechanisms driving P. aeruginosa response to sex steroids hormones. An experiment involving outer membrane vesicles (OMV) extraction combined with Mass spectroscopy was performed to identify differences in P. aeruginosa OMVs proteins produced while being exposed to estradiol and testosterone.

2021-09-08 | PXD019470 | Pride

Transcriptomic remodeling of gastric cells by Helicobacter pylori Outer Membrane Vesicles

Project description:Helicobacter pylori, a bacterium that colonizes the human stomach, like all Gram-negative bacteria spontaneously shed outer membrane vesicles (OMVs). OMVs, which act as a delivery system for bacterial components, are involved in bacterial-host interactions and thus contribute to pathogenesis. In this study, to understand the gene expression changes that human gastric epithelial cells might undergo when exposed to H. pylori-OMVs, we profiled the transcriptomic changes of the MKN74 gastric cell line induced by OMVs compared to control cells and H. pylori-infected cells, using the Ion AmpliSeq™ Transcriptome Human Gene Expression Panel. The top enriched pathways in the OMVs challenge condition included amino acid-related metabolic pathways, mitogen-activated protein kinase signaling, autophagy, and ferroptosis. The cell cycle, DNA replication, and repair pathways were the top diminished pathways. The transcriptomic changes induced by OMVs were largely consistent with those of the bacteria, although often at low expression levels, suggesting that their effects will mostly reinforce those of the bacterium itself. Our data provide a valuable portrayal of the transcriptomic remodeling of gastric cells by H. pylori-OMVs, which can be further dissected regarding the underlying molecular mediators and explored to understand the pathobiology of the full-spectrum of H. pylori-mediated diseases.

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Using small RNA-Seq to identify differential small RNA content of bacterial outer membrane vesicles (OMVs) secreted by Pseudomonas aeruginosa in response to tobramycin exposure

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Haemophilus influenzae Outer membrane vesicles inhalation contributes to steroid-resistant asthma

Project description:Asthma is the most common chronic respiratory disease. Asthma that cannot be well controlled by steroid treatment is called steroid-resistant asthma. Steroid-resistant asthma accounts for only 5% of all asthma cases, but it accounts for 80% of asthma healthcare costs. Nontypeable Haemophilus influenzae (NTHi), as a Gram-negative bacterium, can release outer membrane vesicles (OMVs) and transfer biomolecules to host cells and the external environment by carrying lipopolysaccharides, proteins, peptidoglycans, outer membrane proteins, cell wall components, proteins, nucleic acids, ion metabolites, and signaling molecules. Thus, it plays a role in obtaining nutrition, stress, toxin delivery, adhesion, host immune surveillance evasion, and host immune response regulation. It becomes an essential way in bacterial pathogenesis. To further clarify whether NTHi OMVs could be inhaled to induce steroid-resistant asthma, we isolated and purified NTHi OMVs. In vivo experiments showed that NTHi OMVs could be inhaled and enter airway epithelial cells. Cosensitization with OVA induces steroid-resistant asthma in mice. Furthermore, through high-throughput sequencing, we found that the NTHi OMVs and OVA co-sensitized mice had significantly enriched inflammatory and immune-related signaling pathways, and the transcription and secretion of IL-1β were increased was the potential cause of SRA.

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