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Shotgun proteomic analysis of the unicellular alga Ostreococcus tauri

ABSTRACT: Non soluble fraction, ZT18 rep3

INSTRUMENT(S): LTQ Orbitrap, instrument model, LTQ Orbitrap XL

ORGANISM(S): Ostreococcus Tauri

SUBMITTER: Thierry Le Bihan

PROVIDER: PRD000327 | Pride | 2011-08-31

REPOSITORIES: Pride

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			Action	DRS
	PRIDE_Exp_Complete_Ac_15121.pride.mgf.gz	Mgf
	PRIDE_Exp_Complete_Ac_15121.pride.mztab.gz	Mztab
	PRIDE_Exp_Complete_Ac_15121.xml.gz	Xml
	PRIDE_Exp_Complete_Ac_15122.pride.mgf.gz	Mgf
	PRIDE_Exp_Complete_Ac_15122.pride.mztab.gz	Mztab

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Publications

Shotgun proteomic analysis of the unicellular alga Ostreococcus tauri.

Le Bihan Thierry T Martin Sarah F SF Chirnside Eliane S ES van Ooijen Gerben G Barrios-Llerena Martin E ME O'Neill John S JS Shliaha Pavel V PV Kerr Lorraine E LE Millar Andrew J AJ

Journal of proteomics 20110524 10

Ostreococcus tauri is a unicellular green alga and amongst the smallest and simplest free-living eukaryotes. The O. tauri genome sequence was determined in 2006. Molecular, physiological and taxonomic data that has been generated since then highlight its potential as a simple model species for algae and plants. However, its proteome remains largely unexplored. This paper describes the global proteomic study of O. tauri, using mass spectrometry-based approaches: phosphopeptide enrichment, cellula ...[more]

PMID: 21635980

Similar Datasets

Label free - Shotgun proteomic analysis of the unicellular alga Ostreococcus tauri

Project description:usoluble fraction 24h dark

2012-03-05 | PRD000307 | Pride

Life-cycle and genome of OtV5, a large DNA virus of the pelagic marine unicellular green alga Ostreococcus tauri.

Project description:Large DNA viruses are ubiquitous, infecting diverse organisms ranging from algae to man, and have probably evolved from an ancient common ancestor. In aquatic environments, such algal viruses control blooms and shape the evolution of biodiversity in phytoplankton, but little is known about their biological functions. We show that Ostreococcus tauri, the smallest known marine photosynthetic eukaryote, whose genome is completely characterized, is a host for large DNA viruses, and present an analysis of the life-cycle and 186,234 bp long linear genome of OtV5. OtV5 is a lytic phycodnavirus which unexpectedly does not degrade its host chromosomes before the host cell bursts. Analysis of its complete genome sequence confirmed that it lacks expected site-specific endonucleases, and revealed the presence of 16 genes whose predicted functions are novel to this group of viruses. OtV5 carries at least one predicted gene whose protein closely resembles its host counterpart and several other host-like sequences, suggesting that horizontal gene transfers between host and viral genomes may occur frequently on an evolutionary scale. Fifty seven percent of the 268 predicted proteins present no similarities with any known protein in Genbank, underlining the wealth of undiscovered biological diversity present in oceanic viruses, which are estimated to harbour 200Mt of carbon.

| S-EPMC2386258 | biostudies-literature

Prasinoviruses of the marine green alga Ostreococcus tauri are mainly species specific.

Project description:Prasinoviruses infecting unicellular green algae in the order Mamiellales (class Mamiellophyceae) are commonly found in coastal marine waters where their host species frequently abound. We tested 40 Ostreococcus tauri viruses on 13 independently isolated wild-type O. tauri strains, 4 wild-type O. lucimarinus strains, 1 Ostreococcus sp. ("Ostreococcus mediterraneus") clade D strain, and 1 representative species of each of two other related species of Mamiellales, Bathycoccus prasinos and Micromonas pusilla. Thirty-four out of 40 viruses infected only O. tauri, 5 could infect one other species of the Ostreococcus genus, and 1 infected two other Ostreococcus spp., but none of them infected the other genera. We observed that the overall susceptibility pattern of Ostreococcus strains to viruses was related to the size of two host chromosomes known to show intraspecific size variations, that genetically related viruses tended to infect the same host strains, and that viruses carrying inteins were strictly strain specific. Comparison of two complete O. tauri virus proteomes revealed at least three predicted proteins to be candidate viral specificity determinants.

| S-EPMC3318615 | biostudies-literature

Circadian rhythms study of the unicellular algae Ostreococcus tauri by shotgun proteomics

Project description:The overall set of experiments consist of sampling a culture of the unicellular algae Ostreococcus tauri (wild type) every 4 hours over a period of 24h (6 time points). Each time point was performed with 5 replicate culture flasks, the cultures were grown under a 12:12 hour cycle (light:dark).

2022-12-01 | PXD001734 | Pride

Circadian rhythms study of the unicellular algae Ostreococcus tauri by shotgun proteomics

2022-12-01 | PXD001735 | Pride

An improved genome of the model marine alga Ostreococcus tauri unfolds by assessing Illumina de novo assemblies.

Project description:BACKGROUND: Cost effective next generation sequencing technologies now enable the production of genomic datasets for many novel planktonic eukaryotes, representing an understudied reservoir of genetic diversity. O. tauri is the smallest free-living photosynthetic eukaryote known to date, a coccoid green alga that was first isolated in 1995 in a lagoon by the Mediterranean sea. Its simple features, ease of culture and the sequencing of its 13 Mb haploid nuclear genome have promoted this microalga as a new model organism for cell biology. Here, we investigated the quality of genome assemblies of Illumina GAIIx 75 bp paired-end reads from Ostreococcus tauri, thereby also improving the existing assembly and showing the genome to be stably maintained in culture. RESULTS: The 3 assemblers used, ABySS, CLCBio and Velvet, produced 95% complete genomes in 1402 to 2080 scaffolds with a very low rate of misassembly. Reciprocally, these assemblies improved the original genome assembly by filling in 930 gaps. Combined with additional analysis of raw reads and PCR sequencing effort, 1194 gaps have been solved in total adding up to 460 kb of sequence. Mapping of RNAseq Illumina data on this updated genome led to a twofold reduction in the proportion of multi-exon protein coding genes, representing 19% of the total 7699 protein coding genes. The comparison of the DNA extracted in 2001 and 2009 revealed the fixation of 8 single nucleotide substitutions and 2 deletions during the approximately 6000 generations in the lab. The deletions either knocked out or truncated two predicted transmembrane proteins, including a glutamate-receptor like gene. CONCLUSION: High coverage (>80 fold) paired-end Illumina sequencing enables a high quality 95% complete genome assembly of a compact ~13 Mb haploid eukaryote. This genome sequence has remained stable for 6000 generations of lab culture.

| S-EPMC4378021 | biostudies-literature

Photoperiodic transcriptional responses in Ostreococcus tauri

Project description:Although it is well established the seasonal effect of the photoperiod over the transcriptional expression patterns in plants, this effect has not been studied in microalgae. Here we fill the gap using the model microalgae Ostreococcus tauri.

2023-08-31 | GSE155535 | GEO

The photosystem I supercomplex from a primordial green alga <i>Ostreococcus tauri</i> harbors three light-harvesting complex trimers.

Project description:As a ubiquitous picophytoplankton in the ocean and an early-branching green alga, Ostreococcus tauri is a model prasinophyte species for studying the functional evolution of the light-harvesting systems in photosynthesis. Here, we report the structure and function of the O. tauri photosystem I (PSI) supercomplex in low light conditions, where it expands its photon-absorbing capacity by assembling with the light-harvesting complexes I (LHCI) and a prasinophyte-specific light-harvesting complex (Lhcp). The architecture of the supercomplex exhibits hybrid features of the plant-type and the green algal-type PSI supercomplexes, consisting of a PSI core, an Lhca1-Lhca4-Lhca2-Lhca3 belt attached on one side and an Lhca5-Lhca6 heterodimer associated on the other side between PsaG and PsaH. Interestingly, nine Lhcp subunits, including one Lhcp1 monomer with a phosphorylated amino-terminal threonine and eight Lhcp2 monomers, oligomerize into three trimers and associate with PSI on the third side between Lhca6 and PsaK. The Lhcp1 phosphorylation and the light-harvesting capacity of PSI were subjected to reversible photoacclimation, suggesting that the formation of OtPSI-LHCI-Lhcp supercomplex is likely due to a phosphorylation-dependent mechanism induced by changes in light intensity. Notably, this supercomplex did not exhibit far-red peaks in the 77 K fluorescence spectra, which is possibly due to the weak coupling of the chlorophyll a603-a609 pair in OtLhca1-4.

| S-EPMC10097422 | biostudies-literature

Ostreococcus tauri

Project description:Genome wide rhythmic transcription under light/dark cycles in Ostreococcus tauri

| PRJNA116365 | ENA

Bacteria in Ostreococcus tauri cultures - friends, foes or hitchhikers?

Project description:Marine phytoplankton produce half of the oxygen we breathe and their astounding diversity is just starting to be unraveled. Many microbial phytoplankton are thought to be phototrophic, depending solely on inorganic sources of carbon and minerals for growth rather than preying on other planktonic cells. However, there is increasing evidence that symbiotic associations, to a large extent with bacteria, are required for vitamin or nutrient uptake for many eukaryotic microalgae. Here, we use in silico approaches to look for putative symbiotic interactions by analysing the gene content of microbial communities associated with 13 different Ostreococcus tauri (Chlorophyta, Mamilleophyceae) cultures sampled from the Mediterranean Sea. While we find evidence for bacteria in all cultures, there is no ubiquitous bacterial group, and the most prevalent group, Flavobacteria, is present in 10 out of 13 cultures. Among seven of the microbiomes, we detected genes predicted to encode type 3 secretion systems (T3SS, in 6/7 microbiomes) and/or putative type 6 secretion systems (T6SS, in 4/7 microbiomes). Phylogenetic analyses show that the corresponding genes are closely related to genes of systems identified in bacterial-plant interactions, suggesting that these T3SS might be involved in cell-to-cell interactions with O. tauri.

| S-EPMC4224133 | biostudies-literature

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