Quantitative mass spectrometry reveals a role for the GTPase Rho1p in actin organization on the peroxisome membrane
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ABSTRACT:
INSTRUMENT(S): instrument model
ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)
SUBMITTER: 
Attila Csordas
PROVIDER: PRD000475 | Pride | 2012-07-26
REPOSITORIES: Pride
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Publications
Quantitative mass spectrometry reveals a role for the GTPase Rho1p in actin organization on the peroxisome membrane.
Marelli Marcello M Smith Jennifer J JJ Jung Sunhee S Yi Eugene E Nesvizhskii Alexey I AI Christmas Rowan H RH Saleem Ramsey A RA Tam Yuen Yi C YY Fagarasanu Andrei A Goodlett David R DR Aebersold Ruedi R Rachubinski Richard A RA Aitchison John D JD
The Journal of cell biology 20041213 6
We have combined classical subcellular fractionation with large-scale quantitative mass spectrometry to identify proteins that enrich specifically with peroxisomes of Saccharomyces cerevisiae. In two complementary experiments, isotope-coded affinity tags and tandem mass spectrometry were used to quantify the relative enrichment of proteins during the purification of peroxisomes. Mathematical modeling of the data from 306 quantified proteins led to a prioritized list of 70 candidates whose enrich ...[more]
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