Enhanced Peptide Identification by Electron Transfer Dissociation Using an Improved Mascot Percolator Algorithm
Ontology highlight
ABSTRACT:
INSTRUMENT(S): LTQ Orbitrap, instrument model
ORGANISM(S): Homo Sapiens (human) Escherichia Coli Escherichia Coli (strain K12)
SUBMITTER: 
James Wright
PROVIDER: PRD000544 | Pride | 2013-06-27
REPOSITORIES: Pride
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Enhanced peptide identification by electron transfer dissociation using an improved Mascot Percolator.
Wright James C JC Collins Mark O MO Yu Lu L Käll Lukas L Brosch Markus M Choudhary Jyoti S JS
Molecular & cellular proteomics : MCP 20120406 8
Peptide identification using tandem mass spectrometry is a core technology in proteomics. Latest generations of mass spectrometry instruments enable the use of electron transfer dissociation (ETD) to complement collision induced dissociation (CID) for peptide fragmentation. However, a critical limitation to the use of ETD has been optimal database search software. Percolator is a post-search algorithm, which uses semi-supervised machine learning to improve the rate of peptide spectrum identifica ...[more]
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