Proteomics

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Oil body - LC-MS/MS methods for absolute quantification and identification of proteins associated with chimeric plant oil bodies

ABSTRACT: LC-MS/MS methods for absolute quantification and identification of proteins associated to chimeric plant oil bodies.

INSTRUMENT(S): LTQ Orbitrap, instrument model

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Seed

SUBMITTER: Laurent Gatto  

PROVIDER: PRD000581 | Pride | 2012-03-05

REPOSITORIES: Pride

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Action DRS
PRIDE_Exp_Complete_Ac_19642.pride.mgf.gz Mgf
PRIDE_Exp_Complete_Ac_19642.pride.mztab.gz Mztab
PRIDE_Exp_Complete_Ac_19642.xml.gz Xml
PRIDE_Exp_Complete_Ac_19643.pride.mgf.gz Mgf
PRIDE_Exp_Complete_Ac_19643.pride.mztab.gz Mztab
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LC-MS/MS methods for absolute quantification and identification of proteins associated with chimeric plant oil bodies.

Capuano Floriana F   Bond Nicholas J NJ   Gatto Laurent L   Beaudoin Frédéric F   Napier Johnathan A JA   Benvenuto Eugenio E   Lilley Kathryn S KS   Baschieri Selene S  

Analytical chemistry 20111116 24

Oil bodies (OBs) are plant cell organelles that consist of a lipid core surrounded by a phospholipid monolayer embedded with specialized proteins such as oleosins. Recombinant proteins expressed in plants can be targeted to OBs as fusions with oleosin. This expression strategy is attractive because OBs are easily enriched and purified from other cellular components, based on their unique physicochemical properties. For recombinant OBs to be a potential therapeutic agent in biomedical application  ...[more]

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