Dataset Information

Surfome of different form cells of C. albicans. LJ Chaffin and C. Gil

ABSTRACT: Identification of exposed surface proteins of biofilms, hyphae and yeast form cells of Candida albicans. In vivo trypsin digestion and LC-MS protein identification.

INSTRUMENT(S): instrument model, 4800 Proteomics Analyzer

ORGANISM(S): Candida Albicans Sc5314

TISSUE(S): Hyphal Cell, Yeast, Biofilm

SUBMITTER: Vital Vialas

PROVIDER: PRD000640 | Pride | 2012-10-09

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	PRIDE_Exp_Complete_Ac_21556.pride.mgf.gz	Mgf
	PRIDE_Exp_Complete_Ac_21556.xml.gz	Xml
	PRIDE_Exp_Complete_Ac_21557.pride.mgf.gz	Mgf
	PRIDE_Exp_Complete_Ac_21557.xml.gz	Xml
	PRIDE_Exp_Complete_Ac_21558.pride.mgf.gz	Mgf

Items per page:

1 - 5 of 18

Publications

Cell surface shaving of Candida albicans biofilms, hyphae, and yeast form cells.

Vialás Vital V Perumal Palani P Gutierrez Dolores D Ximénez-Embún Pilar P Nombela César C Gil Concha C Chaffin W LaJean WL

Proteomics 20120801 14

We used a brief trypsin treatment followed by peptide separation and identification using nano-LC followed by off-line MS/MS to identify the surface proteins on live Candida albicans organisms growing in biofilms and planktonic yeast cells and hyphae. One hundred thirty-one proteins were present in at least two of the three replicates of one condition and distributed in various combinations of the three growth conditions. Both previously reported and new surface proteins were identified and thes ...[more]

PMID: 22685022

Similar Datasets

Project description:INTRODUCTION: Combined infections from Candida albicans and Staphylococcus aureus are a leading cause of death in the developed world. Evidence suggests that Candida enhances the virulence of Staphylococcus - hyphae penetrate through tissue barriers, while S. aureus tightly associates with the hyphae to obtain entry to the host organism. Indeed, in a biofilm state, C. albicans enhances the antimicrobial resistance characteristics of S. aureus. The association of these microorganisms is also associated with significantly increased morbidity and mortality. Due to this tight association we hypothesised that metabolic effects were also in evidence.OBJECTIVES: To explore the interaction, we used a novel GC-Orbitrap – based mass spectrometer, the QExactive GC, which combines the high peak capacity and chromatographic resolution of gas chromatography with the sub-ppm mass accuracy of an Orbitrap system. This allows the capability to leverage the widely available electron ionisation libraries for untargeted applications, along with expanding accurate mass libraries and targeted matches based around authentic standards.METHODS: Optimised C. albicans and S. aureus mono- and co-cultured biofilms were analysed in addition to the fresh and spent bacterial growth media.RESULTS: The targeted analysis experiment was based around 36 sugars and sugar phosphates, 22 amino acids and five organic acids. We detected an additional 22 highly scoring compounds from untargeted analysis.CONCLUSION: Many of the results were as expected – rapid consumption of glucose and fructose from the medium regardless of the cell type. We also detected trehalose from the untargeted data, only in medium that contained C. albicans, commensurate with it being a predominantly fungal sugar. Notable from the results is that the pentose phosphate pathway appears to be enhanced in the cells from co-cultured biofilms.

Project description:Candida spp. are commensal opportunistic fungal pathogens that often colonize and infect mucosal surfaces of the human body. Candida, along with other microbes in the microbiota, generally grow as biofilms in a polymicrobial environment. Due to the nature of cellular growth in a biofilm (such as production of a protective extracellular matrix) and the recalcitrance of biofilms, infections involving biofilms are very difficult to treat with antibiotics and perpetuate the cycle of infection. The two most commonly isolated Candida spp. from Candida infections are Candida albicans and Candida glabrata, and the presence of both of these species results in increased patient inflammation and overall biofilm formation. This work aims to investigate the interspecies interactions between C. albicans (Ca) and C. glabrata (Cg) in co-culture through transcriptome analysis over the course of biofilm growth. We report that during co-culture, lipid biosynthesis and transporter genes were significantly modulated in both Ca and Cg. Differentially expressed genes in Ca during co-culture growth included putative transporter genes (C2_02180W_A and C1_09210C_B; up-regulated), amino acid biosynthesis (ARO7; up-regulated most in Ca:Cg 1:3), and lipid-related genes (LIP3 and IPT1; down-regulated). Differentially expressed genes in Cg in co-culture included putative transmembrane transporters (CAGL0H03399g and CAGL0K04609g; up-regulated), an oxidative stress response gene (CAGL0E04114g; down-regulated most in Ca:Cg 1:3), genes involved in the TCA cycle (LYS12 and CAGL0J06402g; down-regulated), and several genes involved in cell wall/membrane biosynthesis (SEC53, GAS2, VIG9; down-regulated). Additionally, confocal microscopy was utilized for membrane lipid analysis between monoculture and co-culture biofilms. Through filipin-stained lipid analysis, we found that there was a significant increase in cell membrane lipid content in Ca:Cg 1:3 biofilms compared to Ca and Ca:Cg 3:1 biofilms. These results suggest substantial modifications of both cell wall, cell membrane, and transporters in both Ca and Cg during the time course of co-culture growth, which allows for increased biofilm formation and virulence in Candida co-culture biofilms.