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Amine-directed tagging of proteins for mass spectrometry-based analyses

ABSTRACT: Amine-directed tagging of proteins for mass spectrometry-based analyses

INSTRUMENT(S): instrument model, Agilent instrument model

ORGANISM(S): Bos Taurus (bovine)

TISSUE(S): Blood Serum

SUBMITTER: Robert Winkler

PROVIDER: PRD000693 | Pride | 2012-10-10

REPOSITORIES: Pride

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			Action	DRS
	PRIDE_Exp_Complete_Ac_22707.pride.mgf.gz	Mgf
	PRIDE_Exp_Complete_Ac_22707.pride.mztab.gz	Mztab
	PRIDE_Exp_Complete_Ac_22707.xml.gz	Xml
	PRIDE_Exp_Complete_Ac_22708.pride.mgf.gz	Mgf
	PRIDE_Exp_Complete_Ac_22708.pride.mztab.gz	Mztab

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Publications

Lysine-directed staining of proteins for MS-based analyses.

Yasui Matthew T MT Mata-Gómez Marco A MA Winkler Robert R

Electrophoresis 20130106 3

Visualization of proteins and MS-based analyses are elemental tasks in modern biochemistry. Nevertheless, reports about covalent protein dyes and their suitability for subsequent MS experiments remain scarce. In a recent work, we demonstrated that covalent prestaining of proteins with Uniblue A drastically speeds up proteomic workflows. The present study introduces dabsyl chloride as another truly MS-compatible protein stain. Remarkably, although Uniblue A and dabsyl chloride employ different nu ...[more]

PMID: 23151947

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