Proteomics

Dataset Information

0

Determination of amino acid sequence of a common dentex (Dentex dentex) egg yolk vitellogenin B derived protein (17.69 kDa) using liquid chromatography electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS)

ABSTRACT: The 17.62 kDa vitellogenin B derived yolk protein was purified by a specific cation exchange chromatography, subjected to a liquid chromatography electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) and finally identified using short MS/MS protein signatures similar to vitellogenin of a near species from the same family, red seabream, Pagrus major, (Temminck and Schlegel, 1843).

INSTRUMENT(S): LTQ Orbitrap, instrument model

ORGANISM(S): Dentex Dentex (common Dentex)

TISSUE(S): Egg

SUBMITTER: Seyed-Mohammadreza SAMAEE  

PROVIDER: PRD000817 | Pride | 2010-02-12

REPOSITORIES: Pride

Json Xml

Dataset's files

Source:
Action DRS
PRIDE_Exp_Complete_Ac_11573.pride.mgf.gz Mgf
PRIDE_Exp_Complete_Ac_11573.xml.gz Xml
PRIDE_Exp_Complete_Ac_11574.pride.mgf.gz Mgf
PRIDE_Exp_Complete_Ac_11574.xml.gz Xml
PRIDE_Exp_Complete_Ac_11575.pride.mgf.gz Mgf
Items per page:
1 - 5 of 10
altmetric image

Publications

Quantitative composition of vitellogenin-derived yolk proteins and their effects on viability of embryos and larvae of common dentex (Dentex dentex), a marine pelagophil teleost.

Samaee Seyed-Mohammadreza SM   Lahnsteiner Franz F   Giménez Gemma G   Estévez Alicia A   Sarg Bettina B   Lindner Herbert H  

Journal of experimental zoology. Part A, Ecological genetics and physiology 20090801 7

To evaluate the importance of the quantitative composition of vitellogenin (Vtg)-derived egg yolk proteins during initial events of Dentex dentex life, viable eggs were obtained from captive broodstocks. Viability parameters (Vps; egg buoyancy (FR), hatching rate (HR), and larval survival rate (SR) at 0, 3, and 5 days posthatch (dph)) were determined for 70 egg batches. Vtg-derived yolk proteins were purified from the eggs using cation-exchange chromatography, separated and detected by SDS-PAGE,  ...[more]

Similar Datasets

Proteomics Label-free LC-MS/MS analysis of silky fowl egg yolk
2022-05-20 | PXD032674 | Pride
Genomics egg yolk and white
| PRJNA837337 | ENA
Proteomics Proteomics of egg yolk nanovesicles
2022-12-07 | MSV000090860 | MassIVE
Proteomics Egg yolk proteins G. fossarum - High-throughput proteome dynamics for discovery of key proteins in sentinel species: Unsuspected vitellogenins diversity in the crustacean Gammarus fossarum
2016-07-15 | PXD001002 | Pride
Proteomics Cryopreservation and egg yolk medium alter the proteome of ram spermatozoa
2018-04-26 | PXD009331 | Pride
Genomics Isolation, characterization, proteome, miRNAome, and the embryotrophic effects of chicken egg yolk nanovesicles (vitellovesicles)
2023-03-22 | GSE219218 | GEO
Unknown Observation of charged droplets from electrospray ionization (ESI) plumes in API mass spectrometers.
| S-EPMC8410725 | biostudies-literature
Unknown Quantification of Signaling Lipids by Nano-Electrospray Ionization Tandem Mass Spectrometry (Nano-ESI MS/MS).
| S-EPMC3901191 | biostudies-literature
Proteomics Dilute to Enrich for Deep Proteomics: Yolk-Depleted Carrier for a Limited Population of Embryonic (Frog) Cells
2024-05-21 | PXD044787 | Pride
Unknown Electrospray ionization (ESI) fragmentations and dimethyldioxirane reactivities of three diverse lactams having full, half, and zero resonance energies.
| S-EPMC3959984 | biostudies-literature