Dataset Information

Chemical Proteomics Partners identification of Cycloamphilectene

ABSTRACT: The identification of the cycloamphilectene cellular interactome has been performed, through chemical proteomics, to unambiguously define its mechanism of action and thus to understand its pharmacological effect. To achieve this goal, a small bioactive molecule (cycloamphilectene) linked to a matrix through a spacer arm “fishes out” its specific interactors from a cell lysate or a tissue extract. Once eluted, cellular partners are separated by SDS-PAGE and identified by high resolution MS and bioinformatics analysis . MS and MS/MS data were acquired using a Q-TOF Premier mass spectrometer (Waters Corp., Micromass, Manchester, United Kingdom). The five most intense ions were automatically chosen by the MassLynx (4.1) software and fragmented. After mass spectrometric measurements, data were automatically processed by ProteinLynx Global Server software to generate peak lists for protein identifications. Database searches were carried out with MASCOT server. The SwissProt human database (release 2010_11 of 02 Nov 10, 522019 sequences, 184241123 residues) was searched, allowing 2 missed cleavages, carbamidomethyl (C) as fixed modification o and oxidation (M) and phosphorylation (ST) as variable modifications. The peptide tolerance was set to 80 ppm and the MS/MS tolerance to 0.8 Da. The Raw data reported herein contains 4 different chemical proteomics experiments carried out to identify cycloamphilectene cellular partner.

INSTRUMENT(S): Q-Tof Premier

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Luigi Margarucci

LAB HEAD: Prof. Agostino Casapullo

PROVIDER: PXD000106 | Pride | 2018-11-26

REPOSITORIES: Pride

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Publications

N-Formyl-7-amino-11-cycloamphilectene, a marine sponge metabolite, binds to tubulin and modulates microtubule depolymerization.

Margarucci Luigi L Monti Maria Chiara MC Esposito Roberta R Tosco Alessandra A Hamel Ernest E Riccio Raffaele R Casapullo Agostino A

Molecular bioSystems 20140204 4

The importance of protein-small molecule interaction in drug discovery, medicinal chemistry and biology has driven the development of new analytical methods to disclose the whole interactome of bioactive compounds. To accelerate targets discovery of N-formyl-7-amino-11-cycloamphilectene (CALe), a marine bioactive diterpene isolated from the Vanuatu sponge Axinella sp., a chemoproteomic-based approach has been successfully developed. CALe is a potent anti-inflammatory agent, modulating NO and pro ...[more]

PMID: 24493364

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			Action	DRS
	CALeExp1_lane1.raw.zip	Raw
	CALeExp1_lane2.raw.zip	Raw
	CALeExp1_lane3.raw.zip	Raw
	CALeExp1_lane4.raw.zip	Raw
	CALeExp1_lane5.raw.zip	Raw