Dataset Information

Stimulated Human Platelet Lysate: control, PAR1 and PAR4 stimulated

ABSTRACT: LC-MS/MS analysis of Control, PAR1-stimulated and PAR4-stimulated platelet lysate digests. Control lysate digest peptides were labeled with "light", PAR1 lysate digest peptides were labeled with "intermediate" and PAR4 lysate digest peptides were labeled with "high" dimethyl labeled. Labeled digests were mixed in a 1:1:1 ratio, separated by SCX and the resulting (phosphopeptide-enriched) peptide fractions were analyzed by LC-MS/MS.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Platelet, Blood

SUBMITTER: O Bleijerveld

LAB HEAD: O Bleijerveld

PROVIDER: PXD000228 | Pride | 2016-05-11

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	OR4_110719_OB_PAR14_sSCX_fr10.raw	Raw
	OR4_110719_OB_PAR14_sSCX_fr11.raw	Raw
	OR4_110719_OB_PAR14_sSCX_fr12.raw	Raw
	OR4_110719_OB_PAR14_sSCX_fr13.raw	Raw
	OR4_110719_OB_PAR14_sSCX_fr14.raw	Raw

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Publications

Quantitative proteomics analysis reveals similar release profiles following specific PAR-1 or PAR-4 stimulation of platelets.

van Holten Thijs C TC Bleijerveld Onno B OB Wijten Patrick P de Groot Philip G PG Heck Albert J R AJ Barendrecht Arjan D AD Merkx Tesy H TH Scholten Arjen A Roest Mark M

Cardiovascular research 20140428 1

<h4>Aims</h4>Platelets are a natural source of growth factors, cytokines and chemokines, that regulate angiogenesis and inflammation. It has been suggested that differential release of pro- and anti-angiogenic growth factors from platelet α-granules by protease-activated receptors (PAR) 1 and 4 may be important for the regulation of angiogenesis. We aimed to compare the releasates of unstimulated platelets with PAR-1- and PAR-4-stimulated platelets.<h4>Methods and results</h4>The release of β-th ...[more]

PMID: 24776597

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