Dataset Information

V5-tagged LIMD1 in HEK293 cells with or without JNK2 activation

ABSTRACT: The experiment was conducted to identify JNK phosphorylation sites on human LIM domain containing protein-1 (LIMD1). V5-tagged LIMD1 was co-transfected or not with a constitutively active form of JNK2 and purified. The purified proteins were subjected to SDS-PAGE, protease K digestion and MS/MS analysis. The data were searched against Swissprot using Mascot server v2.3. The fixed modification was set to carbamidomethylation of C, and variable modifications were set to oxidation of M, phosphorylation of ST, phosphorylation of Y. Mass values were set as monoisotopic. Peptide mass tolerance was set to +/-10ppm, and MS/MS tolerance was set to +/-0.8Da.

INSTRUMENT(S): LTQ

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture

SUBMITTER: Gongping Sun

LAB HEAD: Gongping Sun

PROVIDER: PXD000384 | Pride | 2014-07-09

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	F001463.dat	Other
	F001465.dat	Other
	PRIDE_Exp_Complete_Ac_30469.pride.mgf.gz	Mgf
	PRIDE_Exp_Complete_Ac_30469.pride.mztab.gz	Mztab
	PRIDE_Exp_Complete_Ac_30469.xml.gz	Xml

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Publications

Ajuba family proteins link JNK to Hippo signaling.

Sun Gongping G Irvine Kenneth D KD

Science signaling 20130910 292

Wounding, apoptosis, or infection can trigger a proliferative response in neighboring cells to replace damaged tissue. Studies in Drosophila have implicated c-Jun amino-terminal kinase (JNK)-dependent activation of Yorkie (Yki) as essential to regeneration-associated growth, as well as growth associated with neoplastic tumors. Yki is a transcriptional coactivator that is inhibited by Hippo signaling, a conserved pathway that regulates growth. We identified a conserved mechanism by which JNK regu ...[more]

PMID: 24023255

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