Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Gene expression analysis of V5 tagged Sox17 expressing ES cells

ABSTRACT: Analysis of the expression of KH2 embryonic stem cells inducibly expressing V5 tagged Sox17 protein. Results provide information on the endodermal gene expression program activated after Sox17 expression in ES cells.

ORGANISM(S): Mus musculus

PROVIDER: GSE43233 | GEO | 2013/04/23

SECONDARY ACCESSION(S): PRJNA185342

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Gene expression analysis of V5 tagged Sox17 expressing ES cells

Project description:Analysis of the expression of KH2 embryonic stem cells inducibly expressing V5 tagged Sox17 protein. Results provide information on the endodermal gene expression program activated after Sox17 expression in ES cells. Total RNA extracted from 48 hours doxycycline induced compaired to non-induced Sox17-V5 expressing ES cells

2013-04-23 | E-GEOD-43233 | biostudies-arrayexpress

ChIP-seq of MOZ (Flag-V5-BIO tagged) in mouse mouse craniofacial development

Project description:Carboxy-terminally tagged MOZ (Flag-V5-BIO tagged) was detected by ChIP-seq using anti-V5 antibody (Sigma, A7345) to precipitate chromatin associated with MOZ

2019-07-23 | GSE134607 | GEO

ChIP-seq for V5 in the mammary tissues of Elf5-V5 mice

Project description:V5 tagged Elf5.

2018-09-07 | GSE119657 | GEO

ChIP-Seq analysis of V5-tagged budding yeast Rif1

Project description:Genomic locations of V5-tagged budding yeast Rif1 (including wilt-type and designer mutants) were analysed by ChIP-Seq. Mutants tested were rif1-7A and rif1-7E, in which Ser/Thr residues in the cluster of SQ/TQ sites were mutated to Ala or Glu, respectively. We also tested tested rif1-∆594, in which the C-terminal 594 amino acids were deleted.

2023-11-19 | E-MTAB-13452 | biostudies-arrayexpress

Effects of the Sox17∆50 mutation on SOX17 function in mouse embryonic biliary development

Project description:To determine the role of specific cis-regulatory elements within the Sox17 endoderm-preferential TSS2 promoter, we generated Sox17∆50 mutant animals and surveyed how this mutation altered Sox17 expression. EPCAM+/CXCR4+ endodermal cells were isolated from E10.5 Sox17∆50/∆50 and Sox17+/+ embryos (n = 1 of each genotype). Posterior foregut and midgut regions of embryos were dissected by removal of embryonic head, heart, tail, limb buds, posterior and dorsal body trunk. Sox17+/+ and Sox17Δ50/Δ50 cell samples were labelled with barcodes from 3'CellPlex Kit (10X Genomics) and subsequently pooled together for single isolation, library preparation and sequencing.

2024-10-31 | E-MTAB-14000 | biostudies-arrayexpress

Gene expression analysis of F9 cells double knock-down for Sox7 and Sox17

Project description:Analysis of the expression of F9 cells after knockdown of Sox7 and Sox17 during their primitive endoderm differnetiation induction with retinoic acid. Results provide information on the endodermal gene expression program regulated by Sox7 and Sox17. Sox7 Sox17 double KD vs. control in F9 cells

2013-04-23 | E-GEOD-43232 | biostudies-arrayexpress

Expression data of human eGFP-SOX17+ endoderm cells

2011-03-15 | GSE26862 | GEO

Gene expression analysis of F9 cells double knock-down for Sox7 and Sox17

2013-04-23 | GSE43232 | GEO

Expression data of human eGFP-SOX17+ endoderm cells

Project description:Using homologous recombination in human ESC, we inserted an enhanced green fluorescent protein (eGFP) transgene into a locus encoding a postulated marker of human endoderm, SOX17 in H9 human embryonic stem cells. This allowed purification of SOX17+ hESC endodermal progeny by fluorescence activated cell sorting (FACS) to generate microarray gene expression profile. Using Wnt3 and Activin to differentiate hSOX17-2 to stage 1 cells, and subsequently FGF10 and cyclopamine to stage 2 cells, we isolated eGFP+ cells by FACS at each stage, performed microarray analysis.

2011-03-15 | E-GEOD-26862 | biostudies-arrayexpress

V5-tagged LIMD1 in HEK293 cells with or without JNK2 activation

Project description:The experiment was conducted to identify JNK phosphorylation sites on human LIM domain containing protein-1 (LIMD1). V5-tagged LIMD1 was co-transfected or not with a constitutively active form of JNK2 and purified. The purified proteins were subjected to SDS-PAGE, protease K digestion and MS/MS analysis. The data were searched against Swissprot using Mascot server v2.3. The fixed modification was set to carbamidomethylation of C, and variable modifications were set to oxidation of M, phosphorylation of ST, phosphorylation of Y. Mass values were set as monoisotopic. Peptide mass tolerance was set to +/-10ppm, and MS/MS tolerance was set to +/-0.8Da.

2014-07-09 | PXD000384 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data