Dataset Information

Pea Seedling Mitochondrial Lysine Acetylome

ABSTRACT: Posttranslational lysine acetylation is believed to occur in all taxa and to affect thousands of proteins. In contrast to the hundreds of mitochondrial proteins reported to be lysine-acetylated in non-plant species, only a handful have been reported from the plant taxa previously examined. To investigate whether this reflects a biologically significant difference or merely a peculiarity of the samples thus far examined, we immunoenriched and analyzed acetylated peptides from highly purified pea seedling mitochondria using mass spectrometry. Our results indicate that a multitude of mitochondrial proteins, involved in a variety of processes, are acetylated in pea seedlings.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Pisum Sativum (garden Pea)

TISSUE(S): Seedling

SUBMITTER: Colin Smith-Hammond

LAB HEAD: Jan A. Miernyk

PROVIDER: PXD000495 | Pride | 2014-05-01

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	PsMito1_6p.6.02D5m5KAcMOxe3per_sn1_5to6aa_.3to7kDa_best6hitsPerqSecondPass-likeRAWsQuanNoPeakFilt.msf	Msf
	PsMito2_6p.6.02D5m5KAcMOxe3per_sn1_5to6aa_.3to7kDa_best6hitsPerqSecondPass-likeRAWsQuanNoPeakFilt.msf	Msf
	PsMito3_6p.6.02D5m5KAcMOxe3per_sn1_5to6aa_.3to7kDa_best6hitsPerqSecondPass-likeRAWsQuanNoPeakFilt.msf	Msf
	enriched_CID-HCD_2.RAW	Raw
	enriched_CID-HCD_2cid.ReAnnDB_5KAcMOx6p.xml	Xml

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Publications

The pea seedling mitochondrial Nε-lysine acetylome.

Smith-Hammond Colin L CL Hoyos Elizabeth E Miernyk Ján A JA

Mitochondrion 20140426

Posttranslational lysine acetylation is believed to occur in all taxa and to affect thousands of proteins. In contrast to the hundreds of mitochondrial proteins reported to be lysine-acetylated in non-plant species, only a handful have been reported from the plant taxa previously examined. To investigate whether this reflects a biologically significant difference or merely a peculiarity of the samples thus far examined, we immunoenriched and analyzed acetylated peptides from highly purified pea ...[more]

PMID: 24780491

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