Dataset Information

Low Molecular Weight Allergens from English Walnut (Juglans Regia)

ABSTRACT: Low molecular weight (LMW) proteins were purified from English walnuts (J. regia cv. Chandler). The LMW proteins were separated by anion exchange chromatography. The anion exchange fractions were analyzed by SDS-PAGE, and protein bands were excised. In gel trypsin digestion was conducted (following reduction with DTT and alkylation with iodoacetamide). Peptides were separated by RP-HPLC and analyzed with an LTQ Orbitrap XL. Peptide and protein identifications were obtained with Mascot.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Juglans Regia (english Walnut)

TISSUE(S): Plant Cell, Seed

SUBMITTER: Melanie Downs

LAB HEAD: E.N. Clare Mills

PROVIDER: PXD000634 | Pride | 2019-11-28

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	MelanieAE10_fm111124_10.mgf	Mgf
	MelanieAE10_fm111124_10.mgf-UniProt-5818.dat	Mgf
	MelanieAE11_fm111124_11.mgf	Mgf
	MelanieAE11_fm111124_11.mgf-UniProt-5820.dat	Mgf
	MelanieAE12_fm111124_12.mgf	Mgf

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Publications

Characterization of low molecular weight allergens from English walnut (Juglans regia).

Downs Melanie L ML Semic-Jusufagic Aida A Simpson Angela A Bartra Joan J Fernandez-Rivas Montserrat M Rigby Neil M NM Taylor Steve L SL Baumert Joseph L JL Mills E N Clare EN

Journal of agricultural and food chemistry 20141120 48

Although English walnut is a commonly allergenic tree nut, walnut allergens have been poorly characterized to date. The objective of this work was to characterize the natural, low molecular weight (LMW) allergens from walnut. A protocol was developed to purify LMW allergens (specifically 2S albumins) from English walnuts. In addition to 2S albumins, a series of peptides from the N-terminal region of the 7S seed storage globulin proprotein were also identified and characterized. These peptides co ...[more]

PMID: 25388987

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