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Phosphoproteomics combined with quantitative 14-3-3-affinity capture identifies SIRT1 and RAI as new regulators of cytosolic dsRNA-induced innate immune response

ABSTRACT: In this study, we have used global phosphoproteomic analysis to define phosphorylation-dependent signaling events regulated during RLR stimulation in human keratinocytes. In addition, we used quantitative 14-3-3-affinity capture to identify major target molecules of 14-3-3 regulation.

INSTRUMENT(S): QSTAR

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture, Keratinocyte

SUBMITTER: Tuula Nyman

LAB HEAD: Tuula Nyman

PROVIDER: PXD000670 | Pride | 2014-07-08

REPOSITORIES: Pride

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			Action	DRS
	110623hacat_case_emphasis.xml	Xml
	110623hacat_ctr1.wiff	Wiff
	110623hacat_ctr1.wiff.scan	Wiff
	110623hacat_ctr2.wiff	Wiff
	110623hacat_ctr2.wiff.scan	Wiff

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Publications

Phosphoproteomics combined with quantitative 14-3-3-affinity capture identifies SIRT1 and RAI as novel regulators of cytosolic double-stranded RNA recognition pathway.

Öhman Tiina T Söderholm Sandra S Hintsanen Petteri P Välimäki Elina E Lietzén Niina N MacKintosh Carol C Aittokallio Tero T Matikainen Sampsa S Nyman Tuula A TA

Molecular & cellular proteomics : MCP 20140705 10

Viral double-stranded RNA (dsRNA) is the most important viral structure recognized by cytosolic pattern-recognition receptors of the innate immune system, and its recognition results in the activation of signaling cascades that stimulate the production of antiviral cytokines and apoptosis of infected cells. 14-3-3 proteins are ubiquitously expressed regulatory molecules that participate in a variety of cellular processes, and 14-3-3 protein-mediated signaling pathways are activated by cytoplasmi ...[more]

PMID: 24997996

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14-3-3 affinity capture to identify new targets of 14-3-3 signaling during dsRNA-stimulation in human keratinocytes.

Project description:We have combined two proteomic approaches, phosphoproteomics and 14-3-3 affinity capture, to examine the signaling pathways in which 14-3-3 proteins participate and to identify new targets of 14-3-3 signaling during dsRNA-stimulation.

2014-08-05 | PXD000677 | Pride

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Vitrification of embryos modifies foetal placenta proteome. LCMSMS

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2015-08-11 | PXD001836 | Pride

Comparative Exoprotein Profiling of Different Staphylococcus epidermidis strains Reveals Potential Link between Non-classical Protein Export and Virulence

Project description:This study reports global exoproteome profiling of bovine mastitis strain PM221 and two human strains, commensal-type ATCC12228 and sepsis-associated RP62A to screen for factors underlying adaptation and virulence.

2014-06-04 | PXD000631 | Pride

Rice pollen plasma membrane iTRAQ

Project description:The coordination of pollen tube (PT) growth, guidance and timely growth arrest and rupture mediated by PT-pistil interaction is crucial for the PT to transport sperm cells into ovules for double fertilization. The plasma membrane (PM) represents an important interface for cell–cell interaction, and PM proteins of PTs are pioneers for mediating PT integrity and interaction with pistils. Thus, understanding the mechanisms underlying these events is important for proteomics. Using the efficient aqueous polymer two-phase system and alkali buffer treatment, we prepared high-purity PM from mature and germinated pollen of rice. We used iTRAQ quantitative proteomic methods and identified 1,121 PM-related proteins (PMrPs) (matched to 899 loci); 192 showed differential expression in the two pollen cell types, 119 up- and 73 down-regulated during germination. The PMrP and differentially expressed PMrP sets all showed a functional skew toward signal transduction, transporters, wall remodeling/metabolism and membrane trafficking. Their genomic loci had strong chromosome bias. We found 37 receptor-like kinases (RLKs) from 8 kinase subfamilies and 209 transporters involved in flux of diversified ions and metabolites. In combination with the rice pollen transcriptome data, we revealed that in general, the protein expression of these PMrPs disagreed with their mRNA expression, with inconsistent mRNA expression for 74% of differentially expressed PMrPs. This study, for the first time, identified genome-wide pollen PMrPs, and provided insights into the membrane profile of receptor-like kinases and transporters important for pollen tube growth and interaction with pistils. These pollen PMrPs and their mRNAs showed discordant expression. This work provides novel resource and knowledge to further dissect mechanisms by which pollen or the PT controls PMrP abundance and monitors interactions and ion and metabolite exchanges with female cells in rice.

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An integrative transcriptomic and proteomic analysis of an industrial clavulanic acid-overproducing Streptomyces clavuligerus

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2022-11-02 | PXD032272 | Pride

Proteomic analysis of secreted proteins from Salmonella cells deficient in STnc470

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Measuring C2C12 Myotube Protein Expression Changes in Inflammation using Punch-P

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HIV/HCV co-infection proteomics

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