Proteomics

Dataset Information

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Altered brain pathways following Chikungunya virus infection


ABSTRACT: A kinetic analysis of host proteome modifications in the brain of CHIKV-infected mice sampled before and after the onset of clinical symptoms was performed. The combination of 2D-DIGE and iTRAQ proteomic approaches, followed by mass spectrometry protein identification, revealed 177 significantly differentially expressed proteins. This kinetic analysis was characterized by a dramatic down-regulation of proteins prior the appearance of the clinical symptoms followed by an increase expression of a large part of these proteins in the acute phase of symptoms. Bioinformatic analysis of the protein dataset allowed to identify major biological processes altered during the time course of CHIKV infection such as integrin signaling and cytoskeleton dynamics, endosome machinery and receptor recycling related to virus transport and synapse function, regulation of gene expression, and ubiquitin-proteasome pathway. This work gives new information on putative mechanisms that could be associated with severe neurological CHIKV infection-mediated disease. It also describes possible markers or targets that can be used to develop diagnostic and/or antiviral tools.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Brain

DISEASE(S): Chikungunya

SUBMITTER: Lionel Almeras  

LAB HEAD: Pr. Christophe ROGIER

PROVIDER: PXD000736 | Pride | 2015-07-07

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
chik1-1.raw Raw
chik1-2.raw Raw
chik10-1.raw Raw
chik10-2.raw Raw
chik11-1.raw Raw
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Publications

Kinetic analysis of mouse brain proteome alterations following Chikungunya virus infection before and after appearance of clinical symptoms.

Fraisier Christophe C   Koraka Penelope P   Belghazi Maya M   Bakli Mahfoud M   Granjeaud Samuel S   Pophillat Matthieu M   Lim Stephanie M SM   Osterhaus Albert A   Martina Byron B   Camoin Luc L   Almeras Lionel L  

PloS one 20140311 3


Recent outbreaks of Chikungunya virus (CHIKV) infection have been characterized by an increasing number of severe cases with atypical manifestations including neurological complications. In parallel, the risk map of CHIKV outbreaks has expanded because of improved vector competence. These features make CHIKV infection a major public health concern that requires a better understanding of the underlying physiopathological processes for the development of antiviral strategies to protect individuals  ...[more]

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