Proteomics

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Effects of Campylobacter jejuni CJIE1 prophage on protein expression experiment 3


ABSTRACT: Temperate bacteriophages (prophages) have recently been demonstrated in Campylobacter jejuni. However, what they do there is largely unknown. In the series of studies that are the subject of these submissions we have investigated the relative expression levels of proteins in C. jejuni isolates that differ in the presence or absence of the CJIE1 prophage. At the time of the initial investigations whole genome sequence data were not available for the isolates used, though DNA microarray data indicated that the isolates were very closely related. The overall project was carried out through four separate experiments. In experiment 1, relative levels of protein expression of three isolates carryint the CJIE1 prophage were compared with one lacking the prophage after growth on Mueller-Hinton agar containing blood. Previous work in the scientific literature indicated that growth on medium lacking blood but containing sodium deoxycholate induced the expression of at least some proteins associated with virulence and provided data thought to be of relevance to the virulence of the bacterium. Therefore experiment 2 was done (previous submission) to evaluate in a single 4-plex iTRAQ experiment the effect of sodium deoxycholate on protein expression and whether the presence of the CJIE1 prophage had any effect. The third set of experiments (experiment 3) was done to consolidate the previous observations into a single experiment for a single strain. In three replicate experiments C. jejuni isolate 00-2425 was grown on Mueller Hinton (MH) agar base, MH agar + 10% blood, MH agar containing 0.1% sodium deoxycholate and, to further investigate the nature and extent of the bile response, MH agar containing 2.5% Oxgall.

OTHER RELATED OMICS DATASETS IN: PRJNA213842PRJNA213841PRJNA211797PRJNA214465

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Campylobacter Jejuni Subsp. Jejuni

SUBMITTER: Clifford Clark  

LAB HEAD: Garrett R. Westmacott

PROVIDER: PXD000800 | Pride | 2014-07-31

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
Campy_X1_Fr01.dat Other
Campy_X1_Fr01.raw Raw
Campy_X1_Fr02.dat Other
Campy_X1_Fr02.raw Raw
Campy_X1_Fr03.dat Other
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Publications

The CJIE1 prophage of Campylobacter jejuni affects protein expression in growth media with and without bile salts.

Clark Clifford G CG   Chong Patrick M PM   McCorrister Stuart J SJ   Simon Philippe P   Walker Matthew M   Lee David M DM   Nguy Kimberly K   Cheng Keding K   Gilmour Matthew W MW   Westmacott Garrett R GR  

BMC microbiology 20140319


<h4>Background</h4>The presence of Campylobacter jejuni temperate bacteriophages has increasingly been associated with specific biological effects. It has recently been demonstrated that the presence of the prophage CJIE1 is associated with increased adherence and invasion of C. jejuni isolates in cell culture assays.<h4>Results</h4>Quantitative comparative proteomics experiments were undertaken using three closely related isolates with CJIE1 and one isolate without CJIE1 to determine whether th  ...[more]

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