Proteomics

Dataset Information

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Effects of Campylobacter jejuni CJIE1 prophage on protein expression experiment 4


ABSTRACT: Temperate bacteriophages (prophages) have recently been demonstrated in Campylobacter jejuni. However, what they do there is largely unknown. In the series of studies that are the subject of these submissions we have investigated the relative expression levels of proteins in C. jejuni isolates that differ in the presence or absence of the CJIE1 prophage. At the time of the initial investigations whole genome sequence data were not available for the isolates used, though DNA microarray data indicated that the isolates were very closely related. The overall project was carried out through four separate experiments. Previous work in the scientific literature indicated that growth on medium lacking blood but containing sodium deoxycholate induced the expression of at least some proteins associated with virulence and provided data thought to be of relevance to the virulence of the bacterium. The second set of experiments (experiment 2) compared protein expression in 4-plex iTRAQ experiments using two isolates grown on medium with and without 0.1% sodium deoxycholate to further explore the effects of the CJIE1 prophage, if any, on protein expression. Results from that experiment indicated that there was an unexpectedly large bile response, and that there were differences in expression from the first experiment that may be attributable to the presence of blood in the culture medium in the first experiment, compared with the use of medium without blood in the second experiment. Experiment 3 was therefore designed to compare protein expression of the CJIE1-carrying isolate 00-2425 after growth on medium containing blood, without blood, and without blood but with bile salts. In experiment 4 we grew isolate 00-2426, which lacks prophage CJIE1, on the same four media. The goals were to determine if the differences in protein expression (detection) were similar to those in isolate 00-2425 and if there were any differences that could be attributed to the presence or absence of the CJIE1 prophage.

OTHER RELATED OMICS DATASETS IN: PRJNA213842PRJNA213841PRJNA211797PRJNA214465

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Campylobacter Jejuni Subsp. Jejuni

SUBMITTER: Clifford Clark  

LAB HEAD: Garrett R. Westmacott

PROVIDER: PXD000801 | Pride | 2014-07-31

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
Campy_X2_Fr01.dat Other
Campy_X2_Fr01.raw Raw
Campy_X2_Fr02.dat Other
Campy_X2_Fr02.raw Raw
Campy_X2_Fr03.dat Other
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Publications

The CJIE1 prophage of Campylobacter jejuni affects protein expression in growth media with and without bile salts.

Clark Clifford G CG   Chong Patrick M PM   McCorrister Stuart J SJ   Simon Philippe P   Walker Matthew M   Lee David M DM   Nguy Kimberly K   Cheng Keding K   Gilmour Matthew W MW   Westmacott Garrett R GR  

BMC microbiology 20140319


<h4>Background</h4>The presence of Campylobacter jejuni temperate bacteriophages has increasingly been associated with specific biological effects. It has recently been demonstrated that the presence of the prophage CJIE1 is associated with increased adherence and invasion of C. jejuni isolates in cell culture assays.<h4>Results</h4>Quantitative comparative proteomics experiments were undertaken using three closely related isolates with CJIE1 and one isolate without CJIE1 to determine whether th  ...[more]

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