Proteomics

Dataset Information

0

Quantitative Proteomics Reveals the Role of Protein Phosphorylation in Initial Changes of Soybean Root Tip under Flooding Stress


ABSTRACT: Flooding has a severe negative effect on cultivation in the early stage of soybean growth. To understand the role of protein phosphorylation in early-stage soybean responding to flooding stress, quantitative phosphoproteomic technique was used.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Glycine Max

TISSUE(S): Plant Cell, Root

DISEASE(S): Wounds And Injuries

SUBMITTER: xiaojian Yin  

LAB HEAD: Setsuko Komatsu

PROVIDER: PXD000898 | Pride | 2014-11-13

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
4801.RAW Raw
4801.mgf Mgf
4801.msf Msf
4801.pep.xml Pepxml
4803.RAW Raw
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Publications

Phosphoproteomics reveals the effect of ethylene in soybean root under flooding stress.

Yin Xiaojian X   Sakata Katsumi K   Komatsu Setsuko S  

Journal of proteome research 20141024 12


Flooding has severe negative effects on soybean growth. To explore the flooding-responsive mechanisms in early-stage soybean, a phosphoproteomic approach was used. Two-day-old soybean plants were treated without or with flooding for 3, 6, 12, and 24 h, and root tip proteins were then extracted and analyzed at each time point. After 3 h of flooding exposure, the fresh weight of soybeans increased, whereas the ATP content of soybean root tips decreased. Using a gel-free proteomic technique, a tota  ...[more]

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