Proteomics

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Silkworm testis LC-MS/MS - Proteomics analysis of adult testis from Bombyx mori

ABSTRACT: The development of the testis involves a large number of tissue-specific proteins, possibly because that sperms in it are the most divergent of all cell types. Although the proteome of the larval testis of Bombyx mori has been reported, no comprehensive data on adult testis proteome is available for silkworm. In this study, Liquid chromatography-tandem mass spectrometry was employ to investigate the protein compositions of the adult testis of silkworm. A total of 14431 peptides were identified in the adult testis of B. mori, which were match to 2292 proteins. Thirty-two heat shock proteins constitute a group of most abundant proteins in the adult testis, suggested that they are critical for the development, differentiation and survival of germ cells. Other proteins in this analysis were also involved in testis-specific processes mainly including sperm motility, meiosis, germ cell development, and spermatogenesis.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Bombyx Mori (silk Moth)

TISSUE(S): Testis

SUBMITTER: Yan Zhang  

LAB HEAD: Ping Zhao

PROVIDER: PXD000909 | Pride | 2014-07-28

REPOSITORIES: Pride

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Proteomics analysis of adult testis from Bombyx mori.

Zhang Yan Y   Dong Zhaoming Z   Gu Peiming P   Zhang Weiwei W   Wang Dandan D   Guo Xiaomeng X   Zhao Ping P   Xia Qingyou Q  

Proteomics 20140822 20

The development of the testis involves a large number of tissue-specific proteins, possibly because the sperms in it are the most divergent of all cell types. In this study, LC-MS/MS was employed to investigate the protein compositions of the adult testis of silkworm. A total of 14,431 peptides were identified in the adult testis of Bombyx mori, which were matched to 2292 proteins. Thirty-two HSPs constitute a group of most abundant proteins in the adult testis, suggesting that they are critical  ...[more]

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