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Mycoplasma gallisepticum S6 - Transcriptome analysis reveals novel regulatory mechanisms in a genome-reduced bacterium

ABSTRACT: Avian bacterial pathogen M. gallisepticum is a good model for transcriptional regulation studies due to its small genome and relative simplicity. Here we used RNA-Seq combined with MS-spec proteomics to accurately map coding sequences (CDSs), transcription start sites (TSSs) and transcription terminators (TTs) and decipher their role in stress-induced transcriptional response.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Mycoplasma Gallisepticum S6

SUBMITTER: Ilya Altukhov

LAB HEAD: Govorun Vadim Markovich

PROVIDER: PXD000922 | Pride | 2014-11-12

REPOSITORIES: Pride

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			Action	DRS
	T122.dat-pride.pride.mgf.gz	Mgf
	T122.dat-pride.pride.mztab.gz	Mztab
	T122.dat-pride.xml.gz	Xml
	T122_log_K_C1_IDA_1.wiff	Wiff
	T122_log_K_C1_IDA_1.wiff.scan	Wiff

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Publications

Transcriptome analysis reveals novel regulatory mechanisms in a genome-reduced bacterium.

Mazin Pavel V PV Fisunov Gleb Y GY Gorbachev Alexey Y AY Kapitskaya Kristina Y KY Altukhov Ilya A IA Semashko Tatiana A TA Alexeev Dmitry G DG Govorun Vadim M VM

Nucleic acids research 20141031 21

The avian bacterial pathogen Mycoplasma gallisepticum is a good model for systems studies due to small genome and simplicity of regulatory pathways. In this study, we used RNA-Seq and MS-based proteomics to accurately map coding sequences, transcription start sites (TSSs) and transcript 3'-ends (T3Es). We used obtained data to investigate roles of TSSs and T3Es in stress-induced transcriptional responses. We identified 1061 TSSs at a false discovery rate of 10% and showed that almost all transcr ...[more]

PMID: 25361977

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