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ITRAQ based quantify for pseudomonas

ABSTRACT: The iTRAQ-LC–MS/MS strategy was used for quantitative analysis to reveal the global response of P. aeruginosa SJTD-1 to the stimuli of C18 alkane in this study. As a result, 476 proteins were identified with a significant different expression levels for the growth on n-octadecane, accounting for 8.4% of the 5618 predicted or hypothetical proteins encoded by the strain SJTD-1 genome. These differently expressed proteins were believed to play an important role in multiple biochemical pathways.

INSTRUMENT(S): maXis

ORGANISM(S): Pseudomonas Aeruginosa Sjtd-1

TISSUE(S): Cell Culture

SUBMITTER: Jingli Hou

LAB HEAD: Jingli Hou

PROVIDER: PXD000936 | Pride | 2022-02-22

REPOSITORIES: Pride

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Publications

iTRAQ-based quantitative proteomic analysis of Pseudomonas aeruginosa SJTD-1: A global response to n-octadecane induced stress.

Liu Huan H Sun Wen-Bing WB Liang Ru-Bing RB Huang Li L Hou Jing-Li JL Liu Jian-Hua JH

Journal of proteomics 20150404

N-octadecane, the shortest solid-state alkane, was efficiently consumed by Pseudomonas aeruginosa SJTD-1. To reveal its mechanism, the iTRAQ-LC-MS/MS strategy was applied for quantification of proteins in response to alkane. As a result, 383 alkane-responsive proteins were identified and these proteins could be linked to multiple biochemical pathways. Above all, the level of alkane hydroxylase AlkB2 has been significantly higher in alkane condition. Also, the presence of a putative novel AlmA-li ...[more]

PMID: 25845586

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