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Quantitative phosphoproteomic analysis of TCR signaling

ABSTRACT: To define the physiological properties of TCR signaling, We investigated the global dynamic view of TCR-dependent phosphorylation with the use of thymocytes stimulated with antibodies to CD3 to mimic TCR activation

INSTRUMENT(S): QSTAR

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Primary Cell, T Cell

SUBMITTER: Atsushi Hatano

LAB HEAD: Keiichi I. Nakayama

PROVIDER: PXD000969 | Pride | 2022-02-22

REPOSITORIES: Pride

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			Action	DRS
	20131026_thymocyte_CD3_whole_01.mgf	Mgf
	20131026_thymocyte_CD3_whole_01.pride.mgf.gz	Mgf
	20131026_thymocyte_CD3_whole_02.mgf	Mgf
	20131026_thymocyte_CD3_whole_02.pride.mgf.gz	Mgf
	20131031_thymocyte_CD3_fr1-2.mgf	Mgf

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Publications

Phosphoproteomics analyses show subnetwork systems in T-cell receptor signaling.

Hatano Atsushi A Matsumoto Masaki M Nakayama Keiichi I KI

Genes to cells : devoted to molecular & cellular mechanisms 20160907 10

A key issue in the study of signal transduction is how multiple signaling pathways are systematically integrated into the cell. We have now performed multiple phosphoproteomics analyses focused on the dynamics of the T-cell receptor (TCR) signaling network and its subsystem mediated by the Ca<sup>2+</sup> signaling pathway. Integration of these phosphoproteomics data sets and extraction of components of the TCR signaling network dependent on Ca<sup>2+</sup> signaling showed unexpected phosphoryl ...[more]

PMID: 27600695

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Phosphoproteomic analysis of TCR signaling with inhibitors

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Phosphoproteomic analysis of calcium signaling

Project description:Calcium signaling plays a pivotal role in t cell activation through activation of kinases and phosphatases, but comprehensive analysis to overview calcium-induced phosphorylation has never been performed. To investigate Ca2+-mediated phosphorylation globally, we next undertook a phosphoproteomic analysis of calcium signaling, especially focused on the Ca2+-dependent phosphatase, calcineurin. Normal mouse thymocytes were stimulated in 4 different conditions; 1). DMSO, 2). CsA, 3). IOM, or 4). CsA and IOM, and compared their phosphorylation status.

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Noncanonical pathway for regulation of CCL2 expression by an mTORC1-FOXK1 axis promotes recruitment of tumor-associated macrophages

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2017-11-30 | PXD007996 | Pride

Comparative analysis of the human and mouse placental transcriptome and proteome

Project description:An important question for the use of the mouse as a model for studying human disease is the degree of functional conservation of genetic control pathways from human to mouse. The human and mouse placenta show structural similarities but there have been no systematic attempt to assess their molecular similarities or differences. We built a comprehensive database of protein and microarray data for the highly vascular exchange region micro-dissected from the human and mouse placenta near-term. Abnormalities in this region are associated with two of the most common and serious complications of human pregnancy, maternal preeclampsia (PE) and fetal intrauterine growth restriction (IUGR), each disorder affecting ~5% of all pregnancies. Over 7,000 orthologs were detected with 70% co-expressed and over 80% of genes known to cause placental phenotypes in mouse were co-expressed. These genes form a tight protein-protein interaction network with novel candidate genes likely to be important in placental structure and/or function. The entire data is available as a web-accessible database to guide the informed development of mouse models to study human disease This experiment is now fully represented in NCBI Peptidome database with accession PSE115; http://www.ncbi.nlm.nih.gov/peptidome/search/index.shtml?acc=PSE115 Microdissection of human villous trees and mouse placental labyrinth. Tissues were split for microarray and protein analysis. For protein analysis samples were first fractionated by differential sucrose gradients into mitochrondria, cytosol, microsomes and nuclei. Mitochrondira and neuclei were each extracted by two different methods for soluble and insoluble material. Each subcellular fraction for each tissue was analysed in quintuplet by 9 step 2 dimensional LC/MSMS. This generated a total of 270 mzXML files for each tissue.

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2015-07-22 | E-GEOD-70542 | biostudies-arrayexpress

Packed red blood cells inhibit T-cell activation via ROS-dependent signaling pathways

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Integrated approaches reveal determinants of genome-wide binding and function of the transcription factor Pho4

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