Dataset Information

Comparative proteomics analysis of superior and inferior spikelets in hybrid rice during grain filling and response of inferior spikelets to drought stress using isobaric tags for relative and absolute quantification

ABSTRACT: The biological functions of differently expressed proteins between superior and inferior spikelet grains were investigated based on the isobaric tags for relative and absolute quantification to further clarify the mechanism of rice grain filling at the proteomic level, as well as the response of inferior spikelets to drought dress (-20 kPa or -40 kPa). Compared with superior spikelets, inferior ones had lower sink strength due to the lower sink activities (lower expressions of ADP-glucose pyrophosphorylase, granule-bound starch synthase, starch branching enzyme and pullulanase) and smaller sink sizes (lower abundances of structural proteins). The slower and later grain filling resulted from the weaker decomposition and conversion of photoassimilate and the slower cell division. Moderate drought stress (-20 kPa) promoted the grain filling of inferior spikelets through regulating the proteins associated with photoassimilate supply and conversion. These proteins may be important targets for rice breeding programs that raise the rice yield under drought condition. The findings offer new insights into rice grain-filling and provide theoretical evidences for better quality control and scientific improvement of super rice in practice.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Oryza Sativa (rice)

TISSUE(S): Plant Cell, Seed

SUBMITTER: Minghui Dong

LAB HEAD: Minghui Dong

PROVIDER: PXD001046 | Pride | 2015-08-05

REPOSITORIES: Pride

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Data in support of comparative proteomics analysis of superior and inferior spikelets in hybrid rice during grain filling and response of inferior spikelets to drought stress using isobaric tags for relative and absolute quantification.

Dong Minghui M Gu Junrong J Zhang Li L Chen Peifeng P Liu Tengfei T Deng Jinhua J Lu Haoqian H Han Liyu L Zhao Buhong B

Data in brief 20140812

We provide the raw data for protein and peptide identification and quantization of superior and inferior spikelets in hybrid rice during grain filling. The mass spectrometry proteomics data have been deposited to the Proteome Xchange Consortium via the PRIDE partner repository with the dataset identifier PXD001046. Our data presented here is also related to the article "Comparative proteomics analysis of superior and inferior spikelets in hybrid rice during grain filling and response of inferior ...[more]

PMID: 26217686

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			Action	DRS
	MaxQuant_output.zip	Other
	P13003_1.mgf	Mgf
	P13003_1.raw	Raw
	P13003_2.mgf	Mgf
	P13003_2.raw	Raw