Dataset Information

Proteogenomics of Candida tropicalis – an opportunistic pathogen

ABSTRACT: Candida tropicalis is an opportunistic pathogen which causes candidiasis in immune-compromised individuals. It is one of the members of the non-albicans group of Candida that are known to be azole resistant and is frequently seen in individuals being treated for cancers, HIV-infection and bone-marrow transplant. Although the genome of C. tropicalis was sequenced in the year 2009, the genome annotation has not been supported by experimental validation. In the present study, we have carried out in-depth proteomic profiling of C. tropicalis using high-resolution Fourier transform mass spectrometry and mapped ~44% of the computationally predicted protein-coding genes with peptide level evidence. In addition to identifying 2,740 proteins in the cell lysate of this yeast, we also analysed the proteome of the conditioned media of C. tropicalis culture and identified several unique secreted proteins among a total of 780 proteins. By subjecting the mass spectrometry data derived from cell lysate and conditioned media to proteogenomic analysis, we identified 86 novel genes, 12 novel exons and corrected 49 computationally predicted gene models. To our knowledge, this is the first high-throughput proteomic study to refine the genome annotation of C. tropicalis.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Candida Tropicalis (yeast)

SUBMITTER: Keshava Prasad T. S.

LAB HEAD: T. S. Keshava Prasad

PROVIDER: PXD001071 | Pride | 2017-05-08

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	C_tropicalis_TP_IG_01.raw	Raw
	C_tropicalis_TP_IG_02.raw	Raw
	C_tropicalis_TP_IG_03.raw	Raw
	C_tropicalis_TP_IG_04.raw	Raw
	C_tropicalis_TP_IG_05.raw	Raw

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Publications

Proteogenomics of Candida tropicalis--An Opportunistic Pathogen with Importance for Global Health.

Datta Keshava K KK Patil Arun H AH Patel Krishna K Dey Gourav G Madugundu Anil K AK Renuse Santosh S Kaviyil Jyothi E JE Sekhar Raja R Arunima Aryashree A Daswani Bhavna B Kaur Inderjeet I Mohanty Jyotirmaya J Sinha Ranjana R Jaiswal Sangeeta S Sivapriya S S Sonnathi Yeshwanth Y Chattoo Bharat B BB Gowda Harsha H Ravikumar Raju R Prasad T S Keshava TS

Omics : a journal of integrative biology 20160401 4

The frequency of Candida infections is currently rising, and thus adversely impacting global health. The situation is exacerbated by azole resistance developed by fungal pathogens. Candida tropicalis is an opportunistic pathogen that causes candidiasis, for example, in immune-compromised individuals, cancer patients, and those who undergo organ transplantation. It is a member of the non-albicans group of Candida that are known to be azole-resistant, and is frequently seen in individuals being tr ...[more]

PMID: 27093108

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