Proteomics

Dataset Information

0

Breast Cancer


ABSTRACT: Endocrine resistance in breast cancer is a major clinical problem with poorly understood mechanisms. Mass spectrometry-based proteomics of a clinically-relevant tamoxifen-resistant cell line model identified increased levels of minichromosome maintenance proteins (MCM), including MCM3, as central in cell cycle and DNA replication protein-protein interaction networks associated with tamoxifen resistance. Lowering MCM3 protein expression in tamoxifen-resistant cells restored tamoxifen sensitivity and altered phosphorylation of several cell cycle regulators, such as p53(Ser315, 33), CHK1(Ser317) and cdc25b(Ser323), suggesting that MCM3 activation of important cell cycle-associated proteins overcomes tamoxifen’s anti-proliferative effects. High MCM3 expression in primary tumor tissue from two independent cohorts of ER+ breast cancer patients receiving adjuvant tamoxifen mono-therapy was an independent prognostic marker significantly associated with a shorter recurrence-free survival.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture

DISEASE(S): Breast Cancer

SUBMITTER: Jens Andersen  

LAB HEAD: Henrik J. Ditzel

PROVIDER: PXD001087 | Pride | 2022-02-22

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
20100607MB_WPcyto1.raw Raw
20100607MB_WPcyto10.raw Raw
20100607MB_WPcyto11.raw Raw
20100607MB_WPcyto12.raw Raw
20100607MB_WPcyto2.raw Raw
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Publications


Resistance to endocrine therapy in estrogen receptor-positive (ER<sup>+</sup>) breast cancer is a major clinical problem with poorly understood mechanisms. There is an unmet need for prognostic and predictive biomarkers to allow appropriate therapeutic targeting. We evaluated the mechanism by which minichromosome maintenance protein 3 (MCM3) influences endocrine resistance and its predictive/prognostic potential in ER<sup>+</sup> breast cancer. We discovered that ER<sup>+</sup> breast cancer cel  ...[more]

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