Browse
Submit Data
Databases
API
Help

Dataset Information

396 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Proteomic characterisation of microtubule-dependent modulation of adhesion complexes

ABSTRACT: Investigating the effect of microtubule disruption by nocodazole treatment on isolated adhesion complexes.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Testis, Primary Cell, Fibroblast

SUBMITTER: Daniel Ng

LAB HEAD: Martin James Humphries

PROVIDER: PXD001183 | Pride | 2015-01-30

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	20120309_Bio1_FN_DMSO_Slice1.raw	Raw
	20120309_Bio1_FN_DMSO_Slice10.raw	Raw
	20120309_Bio1_FN_DMSO_Slice11.raw	Raw
	20120309_Bio1_FN_DMSO_Slice12.raw	Raw
	20120309_Bio1_FN_DMSO_Slice13.raw	Raw

Items per page:

1 - 5 of 272

Publications

Microtubule-dependent modulation of adhesion complex composition.

Ng Daniel H J DH Humphries Jonathan D JD Byron Adam A Millon-Frémillon Angélique A Humphries Martin J MJ

PloS one 20141219 12

The microtubule network regulates the turnover of integrin-containing adhesion complexes to stimulate cell migration. Disruption of the microtubule network results in an enlargement of adhesion complex size due to increased RhoA-stimulated actomyosin contractility, and inhibition of adhesion complex turnover; however, the microtubule-dependent changes in adhesion complex composition have not been studied in a global, unbiased manner. Here we used label-free quantitative mass spectrometry-based p ...[more]

PMID: 25526367

Similar Datasets

HeLa and U2OS long term culture adhesion isolations

Project description:Integrin adhesion complexes have been previously isolated and analysed by mass-spectrometry from cells largely spread on matrix proteins over a couple of hours. Here we wished to determine the composition of adhesion complexes from cells cultured for 72 hours under standard cell culture conditions and identify which integrin heterodimers are utilised under these conditions.

2019-06-03 | PXD008645 | Pride

U2OS Adhesion Isolations +/- CytochalasinD and Neomycin

Project description:U2OS cells preferentially utilise integrin alphaV beta5 in adhesion complexes when grown under standard cell culture conditions for 3 days. AlphaV beta5 can be found in both classical 'Focal' adhesions and in novel 'Reticular' adhesions that do not associate with F-actin or other known adhesion protein components. In order to identify components of reticular adhesions, cells were treated with cytochalasinD in order to disrupt F-actin and promote loss of focal adhesions. Remaining reticular adhesion complexes were stabilised with DTBP crosslinking reagent before adhesion complexes were isolated. Preliminary data suggested that depletion of PIP2 by Neomycin treatment would lead to disruption of reticular adhesions preferentially over focal adhesions and so this manipulation was included in these experiments.

2019-06-03 | PXD008680 | Pride

Proteomic analysis of integrin-associated adhesion complexes isolated from cells treated with DMSO or FAK inhibitor

Project description:Human foreskin fibroblast cells were spread on fibronectin (FN) for 1 h and treated with DMSO or FAK inhibitor (3 uM, AZ13256675 (termed AZ675 for short)) for 1 h. Integrin-associated adhesion complexes isolated from these cells were analysed by mass spectrometry to determine changes in adhesion complex composition upon FAK inhibition. As a negative control, complexes were also isolated from cells attached to transferrin (Tf), which allows integrin-independent adhesion via the transferrin receptor.

2016-02-02 | PXD002720 | Pride

Proteomic analysis of integrin adhesion complex disassembly induced by nocodazole

Project description:To explore the dynamics of integrin adhesion complex disassembly, isolated adhesion complexes from U2OS cells were subjected to mass spectrometry based proteomic analysis. U2OS cells were allowed to spread on FN, treated with nocodazole for 4 hours to disrupt microtubules. To coordinate microtubule-induced integrin adhesion complex disassembly nocodazole was subsequently washed out and adhesion complexes isolated at 5, 10 and 15 minutes during this process.

2015-10-14 | PXD002129 | Pride

Mesenchymal stem cell integrin-associated complexes

Project description:The composition and stiffness of the extracellular matrix (ECM) environment that surrounds Multipotent mesenchymal stem cells (MSCs) stem cells dictates transcriptional programming, thereby affecting stem cell lineage decision-making. Cells sense force between themselves and their microenvironment controlling the capability of MSCs to differentiate into adipocytes, osteocytes and chondrocytes. Force sensing is transmitted by integrin receptors and their associated adhesion signalling complexes. To identify regulators of MSC force sensing we sought to catalogue MSC adhesion complex composition. Therefore we isolated integrin-associated adhesion complexes formed in MSCs plated on the ECM ligand fibronectin. We identifed proteins using mass spectrometry that define a MSC specific subset of adhesion complex proteins consisting of key linkages to the actin cytoskeleton together with integrin signalling and force sensing components.

2015-07-20 | PXD001809 | Pride

The tongue squamous carcinoma cell line Cal27 primarily employs integrin α6β4-containing type II hemidesmosomes for adhesion which contribute to anticancer drug sensitivity

Project description:Integrins are heterodimeric cell surface glycoproteins used by cells to bind to the extracellular matrix (ECM) and regulate tumor cell proliferation, migration and survival. A causative relationship between integrin expression and resistance to anticancer drugs has been demonstrated in different tumors, including head and neck squamous cell carcinoma. Using a Cal27 tongue squamous cell carcinoma model, we have previously demonstrated that de novo expression of integrin αVβ3 confers resistance to several anticancer drugs (cisplatin, mitomycin C and doxorubicin) through a mechanism involving downregulation of active Src, increased cell migration and invasion. In the integrin αVβ3 expressing Cal27-derived cell clone 2B1, αVβ5 expression was also increased, but unrelated to drug resistance. To identify the integrin adhesion complex (IAC) components that contribute to the changes in Cal27 and 2B1 cell adhesion and anticancer drug resistance, we isolated IACs from both cell lines. Mass spectrometry (MS)-based proteomics analysis indicated that both cell lines preferentially use integrin α6β4, which is classically found in hemidesmosomes. The anticancer drug resistant cell clone 2B1 demonstrated an increased level of α6β4 accompanied with increased deposition of a laminin-332-containing ECM. Immunofluorescence and electron microscopy demonstrated the formation of type II hemidesmosomes by both cell types. Furthermore, suppression of α6β4 expression in both lines conferred resistance to anticancer drugs. Taken together, our results identify a key role for α6β4-containing type II hemidesmosomes in regulating anticancer drug sensitivity.

2022-02-16 | PXD028810 | Pride

Integrin adhesion complexes formed on different basement membrane ligands

Project description:Adhesion complexes isolated from cells in culture. Podocytes were attached to different extracellular matrix ligands and adhesion complex isolated.

2020-03-09 | PXD017913 | Pride

Proteomic/phosphoproteomic analysis of isolated adhesion complexes

Project description:Combined proteomic and phosphoproteomic analysis of adhesion complexes isolated from cells adherent to the ECM protein fibronectin. A total of 1109 phosphorylation sites were detected, including both known and novel adhesion-induced signalling events.

2016-06-29 | PXD001578 | Pride

Talin1-GFP pulldown in U2OS cells

Project description:In this study, we pull-down human Talin1-GFP and GFP from U2OS cells plated on fibronectin. Binding partners were then analysed using mass-spectrometry.

2021-05-12 | PXD024634 | Pride

Integrin adhesion complex (IAC) analysis from human pancreatic ductal cells (HPDE)

Project description:Pancreatic ductal adenocarcinoma (PDAC) is characterised by an abundant desmoplastic reaction which includes an excess production of extracellular matrix and interacts with integrin adhesion receptors. Integrin adhesion complexes formed by HPDE cells (H6c7), with and without expression of mutant KRas G12V, were isolated and analysed by LC-MSMS

2022-05-20 | PXD027827 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data