Dataset Information

Stadard peptide mixture plus HeLa

ABSTRACT: To test our peak detection and label-free quantitation algorithm, practical applications of AB3D for LC-MS data sets were evaluated using a standard peptide mixture consisting of bovine serum albumin (BSA) peptides with different concentrations, peptides derived from four standard proteins (data set 1), and complex peptide samples derived from HeLa cells and BSA (data set 2).

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Homo Sapiens (human) Bos Taurus (bovine)

TISSUE(S): Serum, Hela Cell

SUBMITTER: Ken Aoshima

LAB HEAD: Ken Aoshima

PROVIDER: PXD001259 | Pride | 2014-12-04

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	mixture_bsa12fmol_n1.mzXML	Mzxml
	mixture_bsa12fmol_n1_Gpm.xml	Xml
	mixture_bsa12fmol_n2.mzXML	Mzxml
	mixture_bsa12fmol_n2_Gpm.xml	Xml
	mixture_bsa12fmol_n3.mzXML	Mzxml

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Publications

A simple peak detection and label-free quantitation algorithm for chromatography-mass spectrometry.

Aoshima Ken K Takahashi Kentaro K Ikawa Masayuki M Kimura Takayuki T Fukuda Mitsuru M Tanaka Satoshi S Parry Howell E HE Fujita Yuichiro Y Yoshizawa Akiyasu C AC Utsunomiya Shin-Ichi S Kajihara Shigeki S Tanaka Koichi K Oda Yoshiya Y

BMC bioinformatics 20141125

<h4>Background</h4>Label-free quantitation of mass spectrometric data is one of the simplest and least expensive methods for differential expression profiling of proteins and metabolites. The need for high accuracy and performance computational label-free quantitation methods is still high in the biomarker and drug discovery research field. However, recent most advanced types of LC-MS generate huge amounts of analytical data with high scan speed, high accuracy and resolution, which is often impo ...[more]

PMID: 25420746

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