Dataset Information
Protein Quantification and Identification from River Bank Grape, Vitis riparia: Comparing SDS-PAGE and FASP-GPF techniques for shotgun proteomic analysis.
Ontology highlight
ABSTRACT:
INSTRUMENT(S): LTQ Orbitrap Velos
ORGANISM(S): Vitis Riparia (frost Grape) (vitis Vulpina) Vitis Vinifera (grape)
TISSUE(S): Plant Cell, Leaf
SUBMITTER: 
Iniga George
LAB HEAD: Paul A Haynes
PROVIDER: PXD001399 | Pride | 2018-04-13
REPOSITORIES: Pride
Dataset's files
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Publications
Protein identification and quantification from riverbank grape, Vitis riparia: Comparing SDS-PAGE and FASP-GPF techniques for shotgun proteomic analysis.
Proteomics 20150622 17
Protein sample preparation optimisation is critical for establishing reproducible high throughput proteomic analysis. In this study, two different fractionation sample preparation techniques (in-gel digestion and in-solution digestion) for shotgun proteomics were used to quantitatively compare proteins identified in Vitis riparia leaf samples. The total number of proteins and peptides identified were compared between filter aided sample preparation (FASP) coupled with gas phase fractionation (GP ...[more]
