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Differentially regulated genes induced in Mycobacterium avium subspecies paratuberculosis by in vitro infection of THP-1 human macrophage cell line

ABSTRACT: Transcriptional profile of Mycobacterium avium subspecies paratuberculosis in in vitro THP-1 (ATCC TIB-202) cell line infection, comparing bacteria grown in host medium and intracellular bacteria recovered from infected THP-1 cells. Two-condition experiment, MAP-K vs. intracellular MAP-THP-1. Biological replicates: 10 controls, 10 intracellular MAP-THP-1, independently grown and harvested. In controls and intracellular MAP-THP-1 cells, good quality RNA samples obtained from each pellet were pooled together and used for microarray experiments to get an average of 10 separate experiments, four replicates per array.

ORGANISM(S): Mycobacterium avium subsp. paratuberculosis

SUBMITTER: Andrea Cossu

PROVIDER: E-GEOD-32242 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Gene expression profiling of Mycobacterium avium subsp. paratuberculosis in simulated multi-stress conditions and within THP-1 cells reveals a new kind of interactive intramacrophage behaviour.

Cossu Andrea A Sechi Leonardo Antonio LA Zanetti Stefania S Rosu Valentina V

BMC microbiology 20120530

<h4>Background</h4>Recent studies have identified in Mycobacterium avium subsp. paratuberculosis (MAP), already known as a pathogen in ruminants, a potential zoonotic agent of some autoimmune diseases in humans. Therefore, considering the possible risk for public health, it is necessary a thorough understanding of MAP's gene expression during infection of human host as well as the identification of its immunogenic and/or virulence factors for the development of appropriate diagnostic and therape ...[more]

PMID: 22646160

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2023-12-04 | PXD047166 | Pride

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