Ontology highlight
ABSTRACT:
INSTRUMENT(S): Q Exactive
ORGANISM(S): Homo Sapiens (human)
TISSUE(S): Epithelial Cell, Cell Culture
DISEASE(S): Colon Cancer
SUBMITTER: Sander Piersma
LAB HEAD: Connie Ramona Jimenez
PROVIDER: PXD001550 | Pride | 2015-04-15
REPOSITORIES: Pride
Items per page: 1 - 5 of 25 |
Journal of proteomics 20150401 Pt B
Robust phosphopeptide enrichment methods with minimal fractionation are required to profile signaling network analysis in cancer cell lines and tissues. We assessed performance of single-shot LC-MS/MS label-free phosphoproteomics using TiOx-based phosphopeptide enrichment and report phosphopeptide identification reproducibility (75.8%), depth of identification (6014-6150 phosphopeptides) and reproducibility of label-free quantification (CV 17.8%). Subsequently, we have profiled the baseline glob ...[more]