Dataset Information

Single muscle fiber proteomics reveals unexpected mitochondrial specialization

ABSTRACT: Mammalian skeletal muscles are composed of multinucleated cells termed slow or fast fibers according to their contractile and metabolic properties. Here we developed a high sensitivity workflow to characterize the proteome of single fibers. Analysis of segments of the same fiber by traditional and unbiased proteomics methods yielded the same subtype assignment. We discovered novel subtype specific features, most prominently mitochondrial specialization of fiber types in substrate utilization. The fiber type resolved proteomes can be applied to a variety of physiological and pathological conditions and illustrate the utility of single cell type analysis for dissecting proteomic heterogeneity.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Skeletal Muscle, Muscle Cell

SUBMITTER: Mario Oroshi

LAB HEAD: Matthias Mann

PROVIDER: PXD001641 | Pride | 2015-02-05

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20130408_EXQ1_MaMu_1107fib_E34_130409140045.raw	Raw
	20130408_EXQ1_MaMu_1107fib_S1_130409184646.raw	Raw
	20130408_EXQ1_MaMu_1107fib_e9_130409091423.raw	Raw
	20130410_EXQ1_MaMu_0407fib_S4.raw	Raw
	20130410_EXQ1_MaMu_1027fib_e4.raw	Raw

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Publications

Single muscle fiber proteomics reveals unexpected mitochondrial specialization.

Murgia Marta M Nagaraj Nagarjuna N Deshmukh Atul S AS Zeiler Marlis M Cancellara Pasqua P Moretti Irene I Reggiani Carlo C Schiaffino Stefano S Mann Matthias M

EMBO reports 20150202 3

Mammalian skeletal muscles are composed of multinucleated cells termed slow or fast fibers according to their contractile and metabolic properties. Here, we developed a high-sensitivity workflow to characterize the proteome of single fibers. Analysis of segments of the same fiber by traditional and unbiased proteomics methods yielded the same subtype assignment. We discovered novel subtype-specific features, most prominently mitochondrial specialization of fiber types in substrate utilization. T ...[more]

PMID: 25643707

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