Proteomics

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Global proteomics analysis reveals a role for histone variant H3.3 in the response to starvation


ABSTRACT: Periodic starvation of animals induces large shifts in metabolism, but may also influence many other cellular systems and can lead to adaption to prolonged starvation conditions. To date, there is limited understanding of how starvation affects gene expression, particularly at the protein level. Here, we have used mass spectrometry‐based quantitative proteomics to identify global changes in the C. elegans proteome due to acute starvation of young adult animals. Measuring changes in abundance of up to 7,000 proteins, we show that acute starvation rapidly alters the levels of hundreds of proteins, many involved in central metabolic pathways, highlighting key regulatory responses. Surprisingly, we also detect changes in the abundance of chromatin‐associated proteins including specific linker histones, histone variants and histone post‐translational modifications associated with the epigenetic control of gene expression. A null mutant for one of these proteins, the histone H3.3 variant HIS‐71, identified a subset of proteins whose abundance no longer varied in response to starvation. This mutant also displayed defects in starvation stress resistance and showed a reduced adult lifespan. To maximise community access to these data, they are presented in an online searchable database, the Encyclopedia of Proteome Dynamics (http://www.peptracker.com/epd/).

INSTRUMENT(S): Q Exactive

ORGANISM(S): Caenorhabditis Elegans

TISSUE(S): Whole Body, Obsolete Cell By Organism

DISEASE(S): Disease Free

SUBMITTER: Vackar Afzal  

LAB HEAD: Angus Lamond

PROVIDER: PXD001723 | Pride | 2015-06-17

REPOSITORIES: Pride

Dataset's files

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Publications

Global Proteomics Analysis of the Response to Starvation in C. elegans.

Larance Mark M   Pourkarimi Ehsan E   Wang Bin B   Brenes Murillo Alejandro A   Kent Robert R   Lamond Angus I AI   Gartner Anton A  

Molecular & cellular proteomics : MCP 20150511 7


Periodic starvation of animals induces large shifts in metabolism but may also influence many other cellular systems and can lead to adaption to prolonged starvation conditions. To date, there is limited understanding of how starvation affects gene expression, particularly at the protein level. Here, we have used mass-spectrometry-based quantitative proteomics to identify global changes in the Caenorhabditis elegans proteome due to acute starvation of young adult animals. Measuring changes in th  ...[more]

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