Dataset Information

Proteomics of EBV-infected lytic and refractory cells

ABSTRACT: Most humans are infected with Epstein-Barr virus (EBV), a cancer-causing virus. While EBV generally persists silently in B lymphocytes, periodic lytic (re-)activation of latent virus is central to its life cycle and to most EBV-related diseases. However, a substantial fraction of EBV-infected B cells and tumor cells in a population is refractory to lytic activation. This resistance to lytic activation directly and profoundly impacts viral persistence and effectiveness of oncolytic therapy for EBV+ cancers. To identify the mechanisms that underlie susceptibility to EBV-lytic activation, we used host protein-expression profiling of separated-lytic and -refractory cells.

OTHER RELATED OMICS DATASETS IN: PRJNA214314MSV000079993

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture, Lymphocyte

DISEASE(S): Lymphoma

SUBMITTER: Jizu Zhi

LAB HEAD: Sumita Bhaduri-McIntosh

PROVIDER: PXD001838 | Pride | 2016-04-27

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	Mudpit_GPM22200000276.mzid.gz	Mzid
	Mudpit_GPM22200000276.mzid_Mudpit_GPM22200000276.MGF	Mzid
	Mudpit_GPM22200000276.mzid_Mudpit_GPM22200000276.pride.mgf.gz	Mzid
	Mudpit_GPM22200000276.pride.mztab.gz	Mztab
	Mudpit_GPM22200000289.mzid.gz	Mzid

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Publications

Cellular STAT3 functions via PCBP2 to restrain Epstein-Barr Virus lytic activation in B lymphocytes.

Koganti Siva S Clark Carissa C Zhi Jizu J Li Xiaofan X Chen Emily I EI Chakrabortty Sharmistha S Hill Erik R ER Bhaduri-McIntosh Sumita S

Journal of virology 20150225 9

<h4>Unlabelled</h4>A major hurdle to killing Epstein-Barr virus (EBV)-infected tumor cells using oncolytic therapy is the presence of a substantial fraction of EBV-infected cells that does not support the lytic phase of EBV despite exposure to lytic cycle-promoting agents. To determine the mechanism(s) underlying this refractory state, we developed a strategy to separate lytic from refractory EBV-positive (EBV(+)) cells. By examining the cellular transcriptome in separated cells, we previously d ...[more]

PMID: 25717101

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