Dataset Information

Label-free protein quantification in freshly ejaculated versus post-mating spermatophores of the noble crayfish Astacus astacus

ABSTRACT: Upon completion of spermatogenesis in crayfish testis, spermatozoa enter the vasa deferentia and are packaged into spermatophores that apparently function in the transfer of spermatozoa from male to the female during mating. In Astacidae and Parastacidae, spermatophores are deposited on the ventral surface of the female, where they remain for several days before initiation of ovulation and subsequent fertilization. This is accompanied by spermatophore morphological changes, suggesting that final maturation of decapod spermatozoa takes place in the seminal receptacle of the female

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Astacus Astacus (noble Crayfish) (astacus Fluviatilis)

TISSUE(S): Sperm, Spermatogonium

SUBMITTER: Hamid Niksirat

LAB HEAD: Professor Peter James

PROVIDER: PXD001929 | Pride | 2015-04-13

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	130222_Hamid_10Aat_01.RAW	Raw
	130222_Hamid_11Aat_01.RAW	Raw
	130222_Hamid_12Aat_01.RAW	Raw
	130222_Hamid_13Aat_01.RAW	Raw
	130222_Hamid_14Aat_01.RAW	Raw

Items per page:

1 - 5 of 17

Publications

Label-free protein quantification in freshly ejaculated versus post-mating spermatophores of the noble crayfish Astacus astacus.

Niksirat Hamid H James Peter P Andersson Liselotte L Kouba Antonín A Kozák Pavel P

Journal of proteomics 20150411

Crayfish spermatophores are deposited on the body surface of the female during mating and remain there for a period of time before fertilization ensues. Post-mating changes in protein expression level in the noble crayfish Astacus astacus spermatophore were quantified. In-gel digestion and high resolution mass spectrometry were used for label-free protein quantification. One hundred twelve proteins were identified in the spermatophore of noble crayfish. After 7 days of storage on the body of the ...[more]

PMID: 25871631

Similar Datasets

Project description:Sturgeons, producers of black caviar, are one of the most valuable wildlife commodities on earth (Pikitch et al., 2005). They appeared approximately 200 million years ago, and are known as living fossils (Bemis et al., 1997). Sturgeon populations dramatically declined as a result of overfishing, poaching and habitat destruction (Birstein, 1993; Billard and Lecointre, 2001; Vidotto et al., 2013). International Union for Conservation of Nature and Natural Resources (IUCN) classified over 85% of sturgeon species as at risk of extinction, more than any other group of species. Therefore, artificial reproduction techniques have been developed for sturgeons to meet the demands of aquaculture and restocking programs. Basic knowledge regarding biology of reproduction such as egg activation and fertilization can help improving the efficiency of artificial reproduction. Fertilization happens when gametes, spermatozoon and egg, are fused together. Egg activation refers to a series of morphological and molecular changes that occur in the egg during and after fertilization. Egg activation is believed to prevent polyspermy, and may also establish a micro-environment to support embryo development (Wong and Wessel, 2006; Niksirat et al., 2015a). Although, earlier researchers have illustrated egg structure and its morphological changes during egg fertilization and activation in sturgeons (Cherr and Clark, 1982, 1985a; Linhart and Kudo, 1997; Debus et al., 2008; Zelazowska, 2010), there is still a lack of molecular knowledge to improve our understanding of this stage of reproduction in these animals. Eggs of sturgeon are released to aqueous environment, where they are fertilized, activated and develop an adhesive layer after contact with freshwater (Cherr and Clark, 1984; Vorobeva and Markov, 1999). Although, egg stickiness is necessary for eggs in nature to help them find a stable position by attaching to substrates such as gravel and stone and increase chance of survival until hatching (Riehl and Patzner, 1998), but can cause high rate of mortality during artificial incubation as a result of attachment of eggs together and subsequent suffocation in limited space of incubators. Using clay suspension in water as an activation medium has been proven to be effective to block egg stickiness in sturgeon eggs (Dettlaff et al., 1993). In recent years, label-free protein quantification techniques have been developed based on the presumption of linear proportionality between peptide mass peak signal intensity and concentration of a given peptide. This approach avoids the limitations and multiple preparation steps of methods such as those based on two-dimensional gel electrophoresis (Niksirat et al., 2015b). The aim of present study was to identify and quantify proteome changes of sterlet eggs during activation in different activation media, water and clay suspension, using label-free protein quantification technique.