Dataset Information

The heparin-binding proteome in nomal pancreas and murine experimental acute pancreatitis, C4PR_LIV

ABSTRACT: Acute pancreatitis (AP) is an acute inflammation of the pancreas, mainly caused by gallstones and alcohol, and driven by changes in communication between cells. Heparin-binding proteins (HBPs) of the plasma membrane and extracellular matrix play a central role in cell communication. Therefore, we used heparin affinity proteomics to identify the extracellular HBPs in mouse normal pancreas (NP) and in a caerulein mouse model of AP. Many new HBPs (460) were discovered more than doubling their total number to 883. A number of the new HBPs are proteins with well-characterised intracellular functions, e.g. NDUFS4, NDUFS6, but which also have a documented extracellular presence with potential ‘moonlighting’ roles. The HBPs form highly interconnected protein-protein interaction networks in both NP and AP, as well as globally. Thus, HBPs may represent the most interconnected set of extracellular proteins and so those with the greatest regulatory potential. HBPs in NP are associated with biological functions such as molecular transport, cellular movement and tissue architecture that underlie pancreatic homeostasis. However, in AP HBPs are additionally associated with processes such as acute phase response signalling, complement system and mitochondrial dysfunction. By virtue of their extracellular location and heparin binding property, HBPs are easily accessible and are potential biomarkers and drug targets in AP.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Pancreas

DISEASE(S): Pancreatitis

SUBMITTER: Philip Brownridge

LAB HEAD: David G Fernig

PROVIDER: PXD001950 | Pride | 2018-12-11

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	PB270912_AP1_500ng_50fmolADH_1.mgf	Mgf
	PB270912_AP1_500ng_50fmolADH_1.mzid.gz	Mzid
	PB270912_AP1_500ng_50fmolADH_1.pride.mgf.gz	Mgf
	PB270912_AP1_500ng_50fmolADH_1.pride.mztab.gz	Mztab
	PB270912_AP1_500ng_50fmolADH_1.raw	Raw

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Publications

The heparin-binding proteome in normal pancreas and murine experimental acute pancreatitis.

Nunes Quentin M QM Su Dunhao D Brownridge Philip J PJ Simpson Deborah M DM Sun Changye C Li Yong Y Bui Thao P TP Zhang Xiaoying X Huang Wei W Rigden Daniel J DJ Beynon Robert J RJ Sutton Robert R Fernig David G DG

PloS one 20190618 6

Acute pancreatitis (AP) is acute inflammation of the pancreas, mainly caused by gallstones and alcohol, driven by changes in communication between cells. Heparin-binding proteins (HBPs) play a central role in health and diseases. Therefore, we used heparin affinity proteomics to identify extracellular HBPs in pancreas and plasma of normal mice and in a caerulein mouse model of AP. Many new extracellular HBPs (360) were discovered in the pancreas, taking the total number of HBPs known to 786. Ext ...[more]

PMID: 31211768

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