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Mytilus galloprovincialis, sperm, LC-MSMS

ABSTRACT: Sperm contains essential proteins for interaction with eggs, however, there are only several sperm proteins reported with important role in fertilization, and gamete proteomics are limited in marine invertebrate species. We present here a sperm proteomic profile of marine mussel Mytilus galloprovincialis. There are 816 proteins were successfully identified by LC-MS/MS based on 1-DE SDS-PAGE. Many of the identifications are relevant to sperm cell physiology and mtDNA functioning. The results will contribute to better understand the proteins involved in fertilization in M. galloprovincialis, as well as the other marine invertebrate species.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Mytilus Galloprovincialis (mediterranean Mussel)

TISSUE(S): Semen

SUBMITTER: Yanjie Zhang

LAB HEAD: QIU Jian-Wen

PROVIDER: PXD001665 | Pride | 2015-07-17

REPOSITORIES: pride

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			Action	DRS
	Mg1-1.mgf	Mgf
	Mg1-1.raw	Raw
	Mg1-2.mgf	Mgf
	Mg1-2.raw	Raw
	Mg1-3.mgf	Mgf

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Publications

Sperm proteome of Mytilus galloprovincialis: Insights into the evolution of fertilization proteins in marine mussels.

Zhang Yanjie Y Mu Huawei H Lau Stanley C K SC Zhang Zhifeng Z Qiu Jian-Wen JW

Proteomics 20150702 23-24

Cataloging the sperm proteome of an animal can improve our understanding of its sperm-egg interaction and speciation, but such data are available for only a few free-spawning invertebrates. This study aimed to identify the sperm proteome of Mytilus galloprovincialis, a free-spawning marine mussel. We integrated public transcriptome datasets by de novo assembly, and applied SDS-PAGE coupled LC-MS/MS analysis to profile the sperm proteome, resulting in the identification of 550 proteins. Comparing ...[more]

PMID: 26046548

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