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Comparative phosphoproteomics reveals the role of AmpC β-lactamase phosphorylation in the clinical imipenem-resistant strain Acinetobacter baumannii SK17 (MaxQuant 1.3 and 1.4)

ABSTRACT: Nosocomial outbreaks of infections caused by multidrug-resistant Acinetobacter baumannii have emerged as a serious threat to human health. The phosphoproteomics of pathogenic bacteria have been investigated for their role in virulence regulation networks. In this study, we analyzed the phosphoproteomics of two clinical isolates of A. baumannii: imipenem-sensitive strain SK17-S and -resistant strain SK17-R.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Acinetobacter Baumannii

TISSUE(S): Prokaryotic Cell, Cell Culture

SUBMITTER: Juo-hsin Lai

LAB HEAD: Juohsin Lai

PROVIDER: PXD002033 | Pride | 2015-10-27

REPOSITORIES: Pride

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			Action	DRS
	17R-1.RAW	Raw
	17R-3.RAW	Raw
	17R-5.RAW	Raw
	17R-7.RAW	Raw
	17R-FT-1.RAW	Raw

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Publications

Comparative Phosphoproteomics Reveals the Role of AmpC β-lactamase Phosphorylation in the Clinical Imipenem-resistant Strain Acinetobacter baumannii SK17.

Lai Juo-Hsin JH Yang Jhih-Tian JT Chern Jeffy J Chen Te-Li TL Wu Wan-Ling WL Liao Jiahn-Haur JH Tsai Shih-Feng SF Liang Suh-Yuen SY Chou Chi-Chi CC Wu Shih-Hsiung SH

Molecular & cellular proteomics : MCP 20151023 1

Nosocomial infectious outbreaks caused by multidrug-resistant Acinetobacter baumannii have emerged as a serious threat to human health. Phosphoproteomics of pathogenic bacteria has been used to identify the mechanisms of bacterial virulence and antimicrobial resistance. In this study, we used a shotgun strategy combined with high-accuracy mass spectrometry to analyze the phosphoproteomics of the imipenem-susceptible strain SK17-S and -resistant strain SK17-R. We identified 410 phosphosites on 24 ...[more]

PMID: 26499836

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