Dataset Information

Profiling of cow urinary proteins using various extraction methods reveals more than 1550 proteins

ABSTRACT: Urine can help in diagnosis of different diseases including cancer and other patho-physiological conditions. Urine proteome studies have been mainly human- centric. No information is available on urinary proteome from bovine till date. In the present study, we have used 3 protein extraction methods such as ammonium sulphate precipitation, ProteoSpin column and diafiltration method from bovine urine for identification of urinary proteome. The tryptic peptides generated after In-gel and In-solution method were identified using LC/MS/MS (ESI-qTOF) which resulted in identification of 1582 proteins. In-gel trypsin digestion method revealed more protein (1191) in comparison to in-solution digestion method (541). Maximum proteins were identified in ammonium sulphate precipitation method (938) followed by ProteoSpin (606) and diafiltration (444) methods respectively. The profile of the identified proteins were compared with human urinary proteome of which 311 bovine urinary proteins matched with human. An exclusive list of 38 bovine urinary proteins with high protein scores were listed which are absent in human urine. All identified proteins were analyzed according to Gene Ontology which were classified according to cellular component, biological processes and molecular functions. This study reports for the first time an exclusive evidence of more than 1550 proteins in urine of healthy cow donors.

INSTRUMENT(S): maXis

ORGANISM(S): Bos Taurus (bovine)

TISSUE(S): Urine

SUBMITTER: Ashok Kumar Mohanty

LAB HEAD: Ashok Kumar Mohanty

PROVIDER: PXD002038 | Pride | 2023-03-22

REPOSITORIES: Pride

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			Action	DRS
	ProteinAnalysisResults423.mgf	Mgf
	ProteinAnalysisResults423.xml	Xml
	ProteinAnalysisResults514.mgf	Mgf
	ProteinAnalysisResults514.xml	Xml
	ProteinAnalysisResults516.mgf	Mgf

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Publications

Profiling of urinary proteins in Karan Fries cows reveals more than 1550 proteins.

Bathla Shveta S Rawat Preeti P Baithalu Rubina R Yadav Munna Lal ML Naru Jasmine J Tiwari Anurag A Kumar Sudarshan S Balhara Ashok K AK Singh Surender S Chaudhary Suman S Kumar Rajesh R Lotfan Masoud M Behare Pradip P Phulia Sushil K SK Mohanty Tushar K TK Kaushik Jai K JK Nallapeta Shivramaiah S Singh Inderjeet I Ambatipudi Srinivas K SK Mohanty Ashok K AK

Journal of proteomics 20150602 Pt A

Urine is a non-invasive source of biological fluid, which reflects the physiological status of the mammals. We have profiled the cow urinary proteome and analyzed its functional significance. The urine collected from three healthy cows was concentrated by diafiltration (DF) followed by protein extraction using three methods, namely methanol, acetone, and ammonium sulphate (AS) precipitation and Proteo Spin urine concentration kit (PS). The quality of the protein was assessed by two-dimensional g ...[more]

PMID: 26021477

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