Dataset Information

Bank Vole Protein Turnover Study C4PR_LIV

ABSTRACT: We have used dietary administration of stable isotope labelled lysine to assess protein turnover rates for proteins from four tissues in the bank vole, Myodes glareolus. The annotated genome for this species is not available, so protein identification was attained through cross-species matching to the mouse. For proteins for which confident identifications were derived, the pattern of lysine incorporation over 40d was used to define the rate of synthesis of individual proteins in the four tissues. The data were heavily filtered to retain a very high quality data-set of turnover rates for 1088 proteins. Comparative analysis of the four tissues revealed different median rates of degradation (kidney: 0.099 per day; liver 0.136 per day; heart, 0.054 per day and skeletal muscle, 0.035 per day). These data were compared with protein degradation rates from other studies on intact animals or from cells in culture.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Myodes Glareolus (bank Vole) (clethrionomys Glareolus) Mus Musculus (mouse)

TISSUE(S): Heart, Skeletal Muscle, Liver, Kidney

SUBMITTER: Dean Hammond

LAB HEAD: Robert John Beynon

PROVIDER: PXD002054 | Pride | 2016-02-09

REPOSITORIES: Pride

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Publications

Proteome Dynamics: Tissue Variation in the Kinetics of Proteostasis in Intact Animals.

Hammond Dean E DE Claydon Amy J AJ Simpson Deborah M DM Edward Dominic D Stockley Paula P Hurst Jane L JL Beynon Robert J RJ

Molecular & cellular proteomics : MCP 20160201 4

Understanding the role of protein turnover in the maintenance of proteostasis requires accurate measurements of the rates of replacement of proteins in complex systems, such as intact animals. Moreover, any investigation of allometric scaling of protein turnover is likely to include species for which fully annotated proteomes are not available. We have used dietary administration of stable isotope labeled lysine to assess protein turnover rates for proteins from four tissues in the bank vole,Myo ...[more]

PMID: 26839000

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			Action	DRS
	12067_Day1.mgf	Mgf
	12067_Day1.mzid.gz	Mzid
	12067_Day1.pride.mgf.gz	Mgf
	12067_Day1.pride.mztab.gz	Mztab
	12067_Day1.raw	Raw