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Arabidopsis col and rglg iron deficiency response

ABSTRACT: To dissect the mechanisms that control and mediate cellular iron homeostasis, we conducted quantitative high-resolution iTRAQ proteomics and microarray-based transcriptomic profiling of iron-deficient Arabidopsis thaliana plants. Proteomic and transcriptomic profiling of Arabidopsis Col and RING DOMAIN LIGASE1 (RGLG1) and RING DOMAIN LIGASE2 (RGLG2) double mutation in response to iron deficiency were conducted. This integrative analysis provides a detailed catalog of post-transcriptionally regulated proteins and allows the concept of a chiefly transcriptionally regulated iron deficiency response to be revisited.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Root, Leaf

SUBMITTER: I-Chun Pan

LAB HEAD: Wolfgang Schmidt

PROVIDER: PXD002126 | Pride | 2015-08-11

REPOSITORIES: Pride

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			Action	DRS
	Leaves.pep.xml	Pepxml
	Leaves.prot.xml	Xml
	Leaves_Bio1-3_peak_list.mzML.gz	Mzml
	Leaves_Bio1.msf.gz	Msf
	Leaves_Bio1_raw.zip	Other

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Publications

Post-Transcriptional Coordination of the Arabidopsis Iron Deficiency Response is Partially Dependent on the E3 Ligases RING DOMAIN LIGASE1 (RGLG1) and RING DOMAIN LIGASE2 (RGLG2).

Pan I-Chun IC Tsai Huei-Hsuan HH Cheng Ya-Tan YT Wen Tuan-Nan TN Buckhout Thomas J TJ Schmidt Wolfgang W

Molecular & cellular proteomics : MCP 20150807 10

Acclimation to changing environmental conditions is mediated by proteins, the abundance of which is carefully tuned by an elaborate interplay of DNA-templated and post-transcriptional processes. To dissect the mechanisms that control and mediate cellular iron homeostasis, we conducted quantitative high-resolution iTRAQ proteomics and microarray-based transcriptomic profiling of iron-deficient Arabidopsis thaliana plants. A total of 13,706 and 12,124 proteins was identified with a quadrupole-Orbi ...[more]

PMID: 26253232

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