Proteomics

Dataset Information

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Systems-wide analysis of manganese deficiency-induced changes in gene activity of Arabidopsis roots


ABSTRACT: In this study, we undertook a comprehensive quantitative proteomic profiling of Arabidopsis roots from plants grown on Mn-deplete media, aiming at dissecting and cataloging the responses of Arabidopsis roots to Mn deficiency. We used LC-Q Exactive Orbitrap mass spectrometer to compile a near complete inventory of the Arabidopsis root proteome. We identified several novel components of cellular Mn homeostasis and show that many putatively important changes in response to Mn deficiency are only evident at one of the omics levels under investigation.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Root

SUBMITTER: Tuan-Nan Wen  

LAB HEAD: Wolfgang Schmidt

PROVIDER: PXD003309 | Pride | 2018-10-26

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
Mn_Arab_iTRAQ_Bio1.msf.gz Msf
Mn_Arab_iTRAQ_Bio1_raw_mzML.rar Other
Mn_Arab_iTRAQ_Bio2.msf.gz Msf
Mn_Arab_iTRAQ_Bio2_raw_mzML.rar Other
Mn_Arab_iTRAQ_Bio3.msf.gz Msf
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Publications

Systems-wide analysis of manganese deficiency-induced changes in gene activity of Arabidopsis roots.

Rodríguez-Celma Jorge J   Tsai Yi-Hsiu YH   Wen Tuan-Nan TN   Wu Yu-Ching YC   Curie Catherine C   Schmidt Wolfgang W  

Scientific reports 20161102


Manganese (Mn) is pivotal for plant growth and development, but little information is available regarding the strategies that evolved to improve Mn acquisition and cellular homeostasis of Mn. Using an integrated RNA-based transcriptomic and high-throughput shotgun proteomics approach, we generated a comprehensive inventory of transcripts and proteins that showed altered abundance in response to Mn deficiency in roots of the model plant Arabidopsis. A suite of 22,385 transcripts was consistently  ...[more]

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